discontinued

DM103 Progesterone receptor antibody

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Quick Overview

Mouse anti Human Progesterone receptor 1A6

DM103

Product Description for Progesterone receptor

Mouse anti Human Progesterone receptor 1A6.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Progesterone receptor

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR
Presentation Supernatant
Reactivity Hu
Applications C, ICC/IF, P
Clonality Monoclonal
Clone 1A6
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06401
Immunogen:
Synthetic peptide
GeneID:
5241
Application Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections after mivrowave pretreatment (See Protocols below).
Working Dilutions: 1/5–1/10 for Immunohistochemistry.
Dilution Buffer: Dilute immediately with PBS or TBS.
Positive Control: Human breast carcinoma.
Background Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N-terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap.
Protocols Application on Paraffin Sections
- Fixation: Small tissue blocks should be fixed as soon as possible after removal, in 10% buffered formalin. The fixation time should not exceed 24 hours. During the embedding process temperatures must be kept below 60°C.
- Mount sections on slides coated with an appropriate medium (Vectabond, APES) at 37°C overnight followed by drying at 56°C for 60 minutes.
- Deparaffinize sections and rehydrate to distilled water.

Antigen Retrieval
- Place slides in a coplin jar containing 0.01 M Sodium Citrate buffer, pH 6.0.
- Incubate for 3 x 5 minutes in a microwave oven (750W) with 1 min intervals.
- Quench sections for 30 min at room temperature. - Rinse for 30 min in PBS.
- Block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol).
- Apply (approximately diluted) Receptor Antibody.
- Continue immunohistochemical staining procedure with conjugate and substrate reaction.
General Readings
  1. Snyders MP et al. Estrogen and progesterone receptor immunohistochemistry in human hyperplastic and neoplastic endometrium. J Pathol 166:171-177 (1992)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Culture Supernatant containing 0.09% Sodium Azide as preservative.
Supernatant
Specificity
Specificity:
The antibody specifically stains tumor cell nuclei, no cytoplasmic staining. The antibody is directed against an epitope at the C-terminal part of the Human Progesterone Receptor (A/B region).
The level of Progesterone and Estrogene receptor protein content in breast cancer tissue is an important parameter for prognosis and treatment.
Species:
Human.
Gene ID 5241
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Progesterone receptor (7 products)

Catalog No. Species Pres. Purity   Source  

Progesterone receptor

Progesterone receptor Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human Purified E. coli
  Acris Antibodies GmbH

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Progesterone receptor

Progesterone receptor Human
  Abnova Taiwan Corp.

Positive controls for Progesterone receptor (1 products)

Catalog No. Species Pres. Purity   Source  

PGR overexpression lysate

PGR overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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