AP00523PU-N Prohibitin antibody

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Rabbit anti Chicken, Human, Mouse, Porcine, Rat Prohibitin

Product Description for Prohibitin

Rabbit anti Chicken, Human, Mouse, Porcine, Rat Prohibitin.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Prohibitin

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DP3115
Presentation Purified
Reactivity Chk, Hu, Ms, Por, Rt
Applications ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified recombinant rat prohibitin protein.
Application Immunofluorescence. Immunoprecipitation (Native and denatured): use Protein A and Ab at 5-10 µg/mg protein lysate. Western Blotting (5-10 µ/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 5-10 µg/ml for 30 minutes at RT; REQUIRES boiling tissue sections in 10mM citrate buffer,pH 6.0; Recommended positive control: A427, MAD109 Cells, Heart.
Background Prohibitin is an evolutionary conserved protein located in the inner membrane of mitochondria. Prohibitin appears to be a very reliable marker of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
Concentration 1.0 mg/ml
General Readings
  1. Sato, T., et al. Cancer Res.1992, 52:1643-1646.
  2. Terashima, M., et al. EMBO J 1994, 13:3782-92.
  3. Nuell, M.J., et al. Mol. Cell. Biol. 11:1372-1381
Storage Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.
Protein A affinity purified immunoglobulin fraction
Buffer System:
10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
This antibody is very usefull as marker for Mitochondria. Cellular Localization: cytoplasmic. Mol. Wt of Antigen: 30 kD.
Human, Pig, Rat, Mouse, and Chicken.

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