DM323-05 Prohibitin antibody

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Mouse anti Chicken, Human, Mouse, Porcine, Rat Prohibitin II-14-10


Product Description for Prohibitin

Mouse anti Chicken, Human, Mouse, Porcine, Rat Prohibitin II-14-10.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Prohibitin

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Chk, Hu, Ms, Por, Rt
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone II-14-10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

BALB/C mice were injected with purified recombinant rat prohibitin protein.

Format: This antibody is supplied as purified immunoglobulin fraction containing 0.05% sodium azide as preservative.
Application Immunohistochemistry on paraffin embedded sections. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Immunoprecipitation. Immunofluorescence. Western Blot.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
General Readings
  1. McClung et al. Exp Gerontol 30 (2): 99, 1995.
  2. Sato et al. Can
Storage Store the antibody at 4-8 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
This antibody is specific to 30 kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression. This antibody cross reacts with mouse, rat and pig. Positive Control: Heart. Cellular Localization: Cytoplasmic.

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