discontinued

DP3115 Prohibitin antibody

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Rabbit anti Chicken, Human, Mouse, Porcine, Rat Prohibitin

DP3115

Product Description for Prohibitin

Rabbit anti Chicken, Human, Mouse, Porcine, Rat Prohibitin.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Prohibitin

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Reactivity Chk, Hu, Ms, Por, Rt
Applications ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Purified recombinant rat prohibitin protein.

Format: This antibody is supplied as liquid, Protein A affinity purified immunoglobulin fraction in 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Application Immunofluorescence. Immunoprecipitation (5-10 µg/mg protein lysate; Native and denatured; Use Protein A). Western Blotting (5-10 µ/ml for 2hrs at RT). Immunohistology (Formalin/paraffin; 5-10 µg/ml for 30 minutes at RT; REQUIRES boiling tissue sections in 10mM citrate buffer,pH 6.0; Recommended positive control: A427, MAD109 Cells, Heart). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
General Readings 1 Sato, T., et al. Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Purified
Specificity
Specificity:
Prohibitin is an evolutionary conserved protein located in the inner membrane of mitochondria. Prohibitin appears to be a very reliable marker of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
Reacts with Human, Pig, Rat, Mouse, and Chicken. Others not tested. Cellular Localization: cytoplasmic.

Mol. Wt of Antigen: 30 kD.

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