SP6379P Protein Kinase C (PKC) theta non pSer345/348 antibody

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Rabbit anti Human Protein Kinase C (PKC) theta non


Product Description for Protein Kinase C (PKC) theta non

Rabbit anti Human Protein Kinase C (PKC) theta non.
Properties: pSer345/348
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Protein Kinase C (PKC) theta non

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Synthetic peptide corresponding to the amino acids 338-352 of human PKC-theta
Property pSer345/348
Application ELISA: 0.1-1.0 µg/ml.
Western Blot: 0.5 to 2.0 µg/ml.
Background Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (α, β, and γ) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (δ, ε, η, and θ isoforms) activated independent of calcium; and 3) atypical PKC (ξ and λ/ι), which are independent of both calcium and DG. nPKC theta consists of 707 amino acid residues and shows the highest sequence similarity to nPKC delta. nPKC theta has a zincfinger- like cysteine-rich sequence (C1 region) and a protein kinase domain sequence (C3 region), both of which are common in all PKC family members. However, nPKC theta lacks a putative Ca+2 binding region (C2 region) that is seen only in conventional PKC subfamily. Analysis of mRNA from various human tissues revealed high-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver.
Concentration 0.2 mg/ml
General Readings
  1. Niino, Y. et al. J. Biol. Chem., Vol. 276 39, 36711 (2001).
  2. Choi, M. et al. J. Biol. Chem., 278 26, 23610 (2003).
  3. Puceat. et al. J. Biol. Chem. 269 24, 16938 (1994).
  4. Osada, S. et al. Mol. Cell. Biol., 3930 12, 9 (1992).
  5. Chang, JD. et al. J. Biol. Chem., 268 19, 14208 (1993).
Storage Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Epitope affinity chromatography
Buffer System:
1x PBS (pH 7.4) containing 0.05 % Sodium Azide
Liquid Ig fraction
Aff - Purified
This antibody reacts specifically to PKC-theta. It was evaluated by western blots and ELISA titration asssays. With ELISA using synthetically prepared PKC-theta peptides, it only recognize PKC-theta and not other kinases or phoshoryhlated PKC-theta (pSer345/348). By the western blot analysis, an immunoreactive band detected at approximately 79 kDa was observed in the whole cell lysates derived from Hela and HEK293.

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