SP6378P Protein Kinase C (PKC) theta pSer345/348 antibody

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Rabbit anti Human Protein Kinase C (PKC) theta pSer345/348

Product Description for Protein Kinase C (PKC) theta pSer345/348

Rabbit anti Human Protein Kinase C (PKC) theta pSer345/348.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Protein Kinase C (PKC) theta pSer345/348

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity Hu
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Synthetic peptide
AA Sequence:
corresponding to human PKC-theta surrounding the phosphorylated residue of Ser345/348.
Application ELISA: 0.1-1.0 µg/ml.
Western Blot: 0.5 to 2 µg/ml.
Background Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (α, β, and γ) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (δ, ε, η, and θ isoforms) activated independent of calcium; and 3) atypical PKC (ξ and λ/ι), which are independent of both calcium and DG. nPKC theta consists of 707 amino acid residues and shows the highest sequence similarity to nPKC delta. nPKC theta has a zincfinger- like cysteine-rich sequence (C1 region) and a protein kinase domain sequence (C3 region), both of which are common in all PKC family members. However, nPKC theta lacks a putative Ca+2 binding region (C2 region) that is seen only in conventional PKC subfamily. Analysis of mRNA from various human tissues revealed high-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver.
Concentration 0.2 mg/ml
General Readings
  1. Niino, Y. et al. J. Biol. Chem., Vol. 276 39, 36711 (2001).
  2. Choi, M. et al. J. Biol. Chem., 278 26, 23610 (2003).
  3. Puceat. et al. J. Biol. Chem. 269 24, 16938 (1994).
  4. Osada, S. et al. Mol. Cell. Biol., 3930 12, 9 (1992).
  5. Chang, JD. et al. J. Biol. Chem., 268 19, 14208 (1993).
Storage Store at 2-8 °C for up to 1 year.
epitope affinity purified
Buffer System:
Supplied in 1X PBS (pH 7.4) containing 0.05 % Sodium Azide.
Aff - Purified
This antibody reacts specifically to PKC-theta (pSer345/348). The antibody was evaluated by western blots and ELISA titration asssays. With ELISA using synthetically prepared PKC-theta (pSer345/348) peptides, it only recognize PKC-theta (pSer345/348) and not other kinases or nonphoshoryhlated PKC-theta (pSer345/348). By the western blot analysis, an immunoreactive band detected at approximately
79 kDa was observed in the whole cell lysates derived from Hela and HEK293.

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