AM00240PU-N Proximal Nephrogenic Renal Antigen (PNRA / gp200) antibody

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Mouse anti Human, Rat Proximal Nephrogenic Renal Antigen (PNRA / gp200) PN-15

Product Description for Proximal Nephrogenic Renal Antigen (PNRA / gp200)

Mouse anti Human, Rat Proximal Nephrogenic Renal Antigen (PNRA / gp200) PN-15.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Proximal Nephrogenic Renal Antigen (PNRA / gp200)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms DM3184P
Presentation Purified
Reactivity Hu, Rt
Applications C, P, WB
Clonality Monoclonal
Clone PN-15
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Microsomal fraction of human renal cortical tissue homogenate.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blotting (see Ref. 1): Reduced and non-reduced.
Immunohistology (Formalin/Paraffin): 2-4 µg/ml 30 Min. RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: Normal Kidney or Renal Cell Carcinoma.
Background In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as, focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty-one other normal tissues do not express similar or cross-reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.
Concentration 0.2 mg/ml
General Readings
  1. Yoshida SO, et. al. Cancer Research, 1989, 49(7):1802-9.
Storage Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.
Protein G affinity purified immunoglobulin fraction
Buffer System:
10 mM PBS, pH 7.4, with 0.09% Sodium Azide as preservative and 0.2% BSA as stabilizer.
This antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. Clone PN-15 may be a useful reagent in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation. Cellular Localization: cell membrane, cytoplasmic. Mol. Wt of Antigen: ~200kD
Human and Rat.

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