DM3184P Proximal Nephrogenic Renal Antigen (PNRA / gp200) antibody

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Mouse anti Human, Rat Proximal Nephrogenic Renal Antigen (PNRA / gp200) PN-15


Product Description for Proximal Nephrogenic Renal Antigen (PNRA / gp200)

Mouse anti Human, Rat Proximal Nephrogenic Renal Antigen (PNRA / gp200) PN-15.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Proximal Nephrogenic Renal Antigen (PNRA / gp200)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3128
Presentation Purified
Reactivity Hu, Rt
Applications C, P, WB
Clonality Monoclonal
Clone PN-15
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Microsomal fraction of human renal cortical tissue homogenate.

Format: This antibody is supplied as liquid, Protein G affinity purified immunoglobulin fraction in 10 mM PBS, pH 7.4, with 0.09% Sodium Azide as preservative and 0.2% BSA as stabilizer.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blotting (1): Reduced & non-reduced.
Immunohistology (Formalin/Paraffin): 2-4 µg/ml 30 Min. RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended positive control: Normal Kidney or Renal Cell Carcinoma.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Yoshida SO, et. al. Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as, focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty-one other normal tissues do not express similar or cross-reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.
Reacts with Human and Rat. Others not tested. Cellular Localization: cell membrane, cytoplasmic.

Mol. Wt of Antigen: ~200kD

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