DM3180 pS2 Estrogen Regulated Protein antibody

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Mouse anti Human pS2 Estrogen Regulated Protein B110.1


Product Description for pS2 Estrogen Regulated Protein

Mouse anti Human pS2 Estrogen Regulated Protein B110.1.
Presentation: Supernatant
Product is tested for Frozen Sections, Paraffin Sections.

Properties for pS2 Estrogen Regulated Protein

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms TFF1
Presentation Supernatant
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone B110.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Synthetic peptide representing a highly antigenic site in the C-terminus end of human pS2 protein. (CFDDTVRGVPWCFYPNTIDVPPEEECEF)
Application Immunohistochemisty 1:25-1:50. This product can be used in formalin-fixed, paraffin embedded tissue sections. For a three-step avidin-biotin complex system, a dilution of 1:25 to 1:50 may be used as a guideline. When using formalin-fixed, paraffin-embedded tissue, enzymatic predigestion with Pronase Reagent (Cat. No. M31) is required. This product is also suitable for use on acetone-fixed frozen sections and cells. Not recomended for Western Blotting. Recommended positive control: Human normal Stomach. About 60% of Breast Carcinoma are positive with pS2. These are also positive with ER & PR receptors. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
General Readings
  1. Piggot NH, et al, J Pathol, 163: 95-104, 1991.
  2. Mori K, et al, J Biochem, 107: 73-76, 1990.
  3. Molina R, et al, Proc. Endocrine Soc, 61 (#148), 1990.
  4. Pallud C, et al, Histopath, 23: 249-256, 1993.
  5. Williams R, et al, Human Pathology, 27: 1259-1266, 1996.
  6. Rio MC, et al, Science, 241: 705-708, 1988.
  7. Abbondanzo SL, et al, Breast Cancer Res. Treat., 16:182, 1990.
  8. Foekens JA, et al, Cancer Res., 50: 3832-3837, 1990.
  9. Schwartz LH, et al, Cancer Res., 51: 624-628, 1993.
  10. Henry JA, et al, Br. J. Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody detects the estrogen-regulated pS2 gene product, a small 6.5 kD, 60 amino-acid polypeptide that belongs to a family of trefoil-shaped growth factors. Cellular Localization: cytoplasmic.

Mol. Wt. of Antigen: 6.5 kD

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