AP55513PU-N Pst1 (N-term) antibody

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Rabbit anti Yeast Pst1

Product Description for Pst1

Rabbit anti Yeast Pst1.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Pst1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Protoplast secreted protein 1
Presentation Aff - Purified
Reactivity Ye
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Molecular weight 49 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
A synthetic peptide from the amino terminus of Yeast Pst1 (AP55513CP-N)
Property N-term
Control peptide AP55513CP-N
Add. information Blocking peptide available: AP55513CP-N
Application Pst1 antibody can be used for detection of Pst1 by Western blot and ELISA at 1 µg/ml.
Background Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (Δoch1 Δmnn1 Δmnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).
Concentration 1.0 mg/ml
General Readings Pardo M, Monteoliva L, Pla J, et al. Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast 1999; 15:459-72.
Pardo M, Monteoliva L, Vázquez P, et al. PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity. Microbiology 2004; 150:4157-70.
Luallen RJ, Lin JQ, Fu H, et al. An Engineered Saccharomyces cerevisiae Strain Binds the Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody 2G12 and Elicits Mannose-Specific gp120-Binding Antibodies. J. Virol. 2008; 82:6447-57.
Luallen RJ, Fu H, Agrawal-Gamse C, et al. A Yeast Glycoprotein Shows High-Affinity Binding to the Broadly Neutralizing Human Immunodeficiency Virus Antibody 2G12 and Inhibits gp120 Interactions with 2G12 and DC-SIGN. J. Virol. 2009; 83:4861-70.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% Sodium Azide as preservative
Liquid purified Ig fraction
Aff - Purified

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