NB120-10345 PSTAIR motif antibody

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Mouse anti Birds, Bovine, Human, Monkey, Mouse, Plant, Rat, Xenopus PSTAIR motif PSTAIR


Product Description for PSTAIR motif

Mouse anti Birds, Bovine, Human, Monkey, Mouse, Plant, Rat, Xenopus PSTAIR motif PSTAIR.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for PSTAIR motif

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ascites
Reactivity Av, Bov, Hu, Mky, Ms, Pl, Rt, Xen
Applications E, IP, WB
Clonality Monoclonal
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA.
Application ELISA, immunoprecipitation, Western Blot, ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.WB: Use at a dilution of 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunoprecipitation, Western Blot
Background The cell cycle is controlled in part by cyclin-dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
General Readings General / background references:Morgan DO Principles of CDK regulation. Nature 374:131-4 (1995). PubMed PMID: 7877684
Mueller PR et al. Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15. Science 270:86-90 (1995). PubMed PMID: 7569953
Hunter T & Pines J Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age. Cell 79:573-82 (1994). PubMed PMID: 7954824
Draetta G Cdc2 activation: the interplay of cyclin binding and Thr161 phosphorylation. Trends Cell Biol 3:287-9 (1993). PubMed PMID: 14731844
Freeman RS & Donoghue DJ Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle. Biochemistry 30:2293-302 (1991). PubMed PMID: 2001361
Storage Store at -20C. Avoid freeze-thaw cycles.
Buffer System:
0.1% Sodium Azide
Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3)., PSTAIR (PSTAIR)
Avian, Bovine, Human, Mouse, Plant, Primate, Rat, Xenopus

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