TA306251 PTRH2 / BIT1 (N-term) antibody

Rabbit Polyclonal Bit1 Antibody

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0.1 mg / €371.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat PTRH2 / BIT1

TA306251

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Product Description for PTRH2 / BIT1

Rabbit anti Human, Mouse, Rat PTRH2 / BIT1.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for PTRH2 / BIT1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y3E5
Immunogen:
Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (N-Terminus) Peptide (AP30152CP-N)
GeneID:
51651
Property N-term
Add. information Blocking peptide available: blocking peptide (AP30152CP-N)
Application ELISA.
Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 – 2 μg/ml.
Immunocytochemistry.
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).
General Readings
  1. Martin SS, Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004 Jul 5;1692(2-3):145-57. PubMed PMID: 15246684.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% sodium azide.
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
Bit1 antibody was raised against a 15 amino acid peptide from near the amino-terminus of human Bit1.
Species:
Human, Mouse, Rat.

Accessory Products

Proteins and/or Positive Controls

Proteins for PTRH2 / BIT1 (6 products)

Catalog No. Species Pres. Purity   Source  

PTRH2 / BIT1 (64-179, His-tag)

PTRH2 / BIT1 Human Purified > 95 % E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

PTRH2 / BIT1 (64-179, His-tag)

PTRH2 / BIT1 Human Purified > 95 % E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

PTRH2 / BIT1

PTRH2 / BIT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PTRH2 / BIT1

PTRH2 / BIT1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PTRH2 / BIT1

PTRH2 / BIT1 Human Purified
  Novus Biologicals Inc.

PTRH2 / BIT1

PTRH2 / BIT1 Human Purified
  Novus Biologicals Inc.

Positive controls for PTRH2 / BIT1 (4 products)

Catalog No. Species Pres. Purity   Source  

PTRH2 293T Cell Transient Overexpression Lysate(Denatured)

PTRH2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

PTRH2 / BIT1 Lysate(Denatured)

PTRH2 / BIT1 Lysate(Denatured)
  Abnova Taiwan Corp.

PTRH2 Lysate

Western Blot: PTRH2 Lysate [NBL1-14990] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PTRH2
  Novus Biologicals Inc.

PTRH2 overexpression lysate

PTRH2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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