NB100-94268 PUMA antibody

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Rabbit anti Human, Mouse PUMA

Product Description for PUMA

Rabbit anti Human, Mouse PUMA.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for PUMA

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Rabbit anti-PUMA polyclonal antibody was raised against a synthetic peptide (PLPRGHRAPEMEPN) corresponding to amino acids 180 to 193 of human PUMA-alpha (1). This sequence is identical between alpha and beta forms of the PUMA proteins.
Add. information Research Areas: Cancer
Application Western Blot, This antibody can be used for detection of PUMA by Western blot at 1 to 2 ug/ml. K562 or 3T3 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form. It is human and mouse reactive., Western Blot 1-2 ug/ml,
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Storage Store at 4C. Do not freeze.
IgG purified
Buffer System:
0.02% Sodium Azide
IgG purified
PUMA bbc3
Human, Mouse

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