NB300-663 Punt antibody

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Rabbit anti Drosophila Punt

Product Description for Punt

Rabbit anti Drosophila Punt.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Punt

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Dros
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide: QLLIKNHTNIDDAKESTNC.Peptides were amidated for conjugation.
Application Immunocytochemistry, immunoprecipitation, Western Blot, immunoprecipitation, Western Blot, Immunocytochemistry Detects a band of approximately 63 kDa. Optimal dilutions and concentrations should be determined by the end user., immunoprecipitation 1:200, Western Blot 1:500, Immunocytochemistry assay dependent
Background Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2). The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor encoded by the thick veins (tkv) gene and the type II receptor encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF-beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required ot block the development of various organelles including salivary glands, these are Mothers against dpp (Mad), Medea (Med) Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development (3). Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp-dependent patterning process. No Dpp-dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling.
Concentration 0.75 mg/ml
General Readings General / background references:Henderson KD et al. Cell fate specification in the Drosophila salivary gland: the integration of homeotic gene function with the DPP signaling cascade. Dev Biol 205:10-21 (1999). PubMed PMID: 9882494
Xie T & Spradling AC decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. Cell 94:251-60 (1998). PubMed PMID: 9695953
Singer MA et al. Signaling through both type I DPP receptors is required for anterior-posterior patterning of the entire Drosophila wing. Development 124:79-89 (1997). PubMed PMID: 9006069
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.02% Sodium Azide
Immunogen affinity purified
Aff - Purified

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