discontinued

NSB214 RAC1 + Cdc42 (pSer71) antibody

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Search for all "RAC1 + Cdc42"

0.1 ml / €380.00

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Rabbit anti Human RAC1 + Cdc42

Product Description for RAC1 + Cdc42

Rabbit anti Human RAC1 + Cdc42.
Properties: (pSer71)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for RAC1 + Cdc42

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic phospho peptide (Human) containing serine 71. The sequence is conserved in human and mouse RAC 1, 2, and 3, and Cdc 42 human, mouse, rat, dog and frog.
Property pSer71
Add. information Research Areas: Cancer
Application Western Blot, WB: Use at a dilution of 1/1000. Detects a band of approximately 23 kDa., Western Blot
Background RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.
General Readings General / background references:Servitja JM et al. Rac1 function is required for Src-induced transformation. Evidence of a role for Tiam1 and Vav2 in Rac activation by Src. J Biol Chem 278:34339-46 (2003). PubMed PMID: 12810717
Sundberg LJ et al. An endogenous inhibitor of focal adhesion kinase blocks Rac1/JNK but not Ras/ERK-dependent signaling in vascular smooth muscle cells. J Biol Chem 278:29783-91 (2003). PubMed PMID: 12782622
Vidal C et al. Cdc42/Rac1-dependent activation of the p21-activated kinase (PAK) regulates human platelet lamellipodia spreading: implication of the cortical-actin binding protein cortactin. Blood 100:4462-9 (2002). PubMed PMID: 12453877
Fukuda T et al. Novel mechanism of regulation of Rac activity and lamellipodia formation by RET tyrosine kinase. J Biol Chem 277:19114-21 (2002). PubMed PMID: 11886862
Kwon T et al. Akt protein kinase inhibits Rac1-GTP binding through phosphorylation at serine 71 of Rac1. J Biol Chem 275:423-8 (2000). PubMed PMID: 10617634
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react.
Species:
Human

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