TA302815 RanBP7 / Importin-7 (C-term) antibody

Goat Polyclonal Antibody against IPO7

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Bovine, Canine, Human, Mouse, Rat RanBP7 / Importin-7

Product Description for RanBP7 / Importin-7

Goat anti Bovine, Canine, Human, Mouse, Rat RanBP7 / Importin-7.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for RanBP7 / Importin-7

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms IMP7, IPO7, Ran-binding protein 7
Presentation Aff - Purified
Reactivity Bov, Can, Hu, Ms, Rt
Applications E, P, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95373
Immunogen:
Synthetic peptide from C-terminus of Human IPO7 (NP_006382.1).
AA Sequence:
C-SSFNFGGPAPGMN
GeneID:
10527
Property C-term
Application Peptide ELISA: 1/4000 (Detection Limit).
Western blot: 1-3 µg/ml. Approx 120kDa band observed in lysates of cell lines A431, Daudi and HeLa (calculated MW of 120kDa according to NP_006382.1). An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide.
Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Background The Importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran binding site of importin beta. Similar to importin beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta like transport receptors, namely importin beta itself, transportin, RanBP5 and Importin 7, directly bind and import ribosomal proteins.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Saijou E, Itoh T, Kim KW, Iemura S, Natsume T, Miyajima A. Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms. J Biol Chem. 2007 Nov 2;282(44):32327-37. Epub 2007 Sep 11. PubMed PMID: 17848547.
General Readings
  1. Görlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, et al. A novel class of RanGTP binding proteins. J Cell Biol. 1997 Jul 14;138(1):65-80. PubMed PMID: 9214382. (Free PMC Article available, 11 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH 7.3 containing of 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Dog, Cow.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects RanBP7/Importin-7 at C-term.

Accessory Products

Proteins and/or Positive Controls

Proteins for RanBP7 / Importin-7 (2 products)

Catalog No. Species Pres. Purity   Source  

RanBP7 / Importin-7

RanBP7 / Importin-7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

RanBP7 / Importin-7

RanBP7 / Importin-7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.
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