TA319831 RAPGEF4 (N-term) antibody

Rabbit Polyclonal EPAC2 Antibody

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0.1 mg / €325.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat RAPGEF4

TA319831

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  • TA319831
  • TA319831

Product Description for RAPGEF4

Rabbit anti Human, Mouse, Rat RAPGEF4.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for RAPGEF4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CGEF2, EPAC 2, EPAC2, Exchange factor directly activated by cAMP 2, Exchange protein directly activated by cAMP 2, Rap guanine nucleotide exchange factor 4, cAMP-GEFII, cAMP-regulated guanine nucleotide exchange factor II
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8WZA2
Immunogen:
15 amino acid synthetic peptide near the amino terminus of human RAPGEF4 / EPAC2 (AP55604CP-N)
GeneID:
11069
Property N-term
Add. information Blocking peptide available: AP55604CP-N
Application Western blot: 1-2 μg/ml.
Positive Control: Rat liver tissue lysate.
Immunohistochemistry on Paraffin section: Start at 2.5 μg/ml.
Background EPAC2, also known as Rap guanine nuclear exchange factor 4 (RAPGEF4) and cAMPGEF-II, is belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins with guanine nucleotide exchange factor. Like the related protein EPAC1, EPAC2 signaling plays a role in numerous cellular processes such as integrin-mediated cell adhesion, muscle contraction, learning and memory, cell proliferation, and inflammation. Recent evidence suggests that EPAC2 induces synapse remodeling and depression, with mutations in its gene being found in patients with autism.
Concentration 1.0 mg/ml
General Readings
  1. Ueno H, Shibasaki T, Iwanaga T, Takahashi K, Yokoyama Y, Liu LM, et al. Characterization of the gene EPAC2: structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform. Genomics. 2001 Nov;78(1-2):91-8. PubMed PMID: 11707077.
  2. Grandoch M, Roscioni SS, Schmidt M. The role of Epac proteins, novel cAMP mediators, in the regulation of immune, lung and neuronal function. Br J Pharmacol. 2010 Jan 1;159(2):265-84. doi: 10.1111/j.1476-5381.2009.00458.x. Epub 2009 Nov 11. PubMed PMID: 19912228. (Free PMC Article available, 4 images available)
  3. Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J. Physiol. 2006; 577:5-15. 
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% Sodium Azide as preservative
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat
Specificity
Specificity:
At least two isoforms of RAPGEF4 / EPAC2 are known to exist. This antibody will detect only the larger isoform.

Accessory Products

Proteins and/or Positive Controls

Proteins for RAPGEF4 (5 products)

Catalog No. Species Pres. Purity   Source  

RAPGEF4 (transcript variant 1)

RAPGEF4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RAPGEF4

RAPGEF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

RAPGEF4

RAPGEF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for RAPGEF4 (2 products)

Catalog No. Species Pres. Purity   Source  

Epac2 Lysate

Western Blot: Epac2 Lysate [NBL1-15153] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for RAPGEF4
  Novus Biologicals Inc.

RAPGEF4 293T Cell Transient Overexpression Lysate(Denatured)

RAPGEF4 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
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