AM32148PU-N Rat Tumors, epithelial origin antibody

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0.1 mg / €810.00
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Mouse anti Rat Rat Tumors, epithelial origin MG1

AM32148PU-N

Product Description for Rat Tumors, epithelial origin

Mouse anti Rat Rat Tumors, epithelial origin MG1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation, Paraffin Sections, Frozen Sections.

Properties for Rat Tumors, epithelial origin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Rat Tumor Marker
Presentation Purified
Reactivity Rt
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone MG1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Immunization of mice with Rat colon adenocarcinoma cells (CC531).
Remarks:
Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001).
Application ELISA. 
Western blotting. 
Flow Cytometry.
Immunoprecipitation. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Tissue:
There is some cross reactivity in thymus and lymph nodes. Citric acid or EDTA pre-treatment is recommended.
Background Common epithelial tumors begin in the surface epithelium of the ovaries and account for about 90% of all ovarian cancers. They are divided into a number of subtypes - including serous, endometrioid, mucinous, and clear cell tumors - that can be further classified as benign (noncancerous) or malignant (cancerous) tumors.
General Readings
  1. Hagenaars M, Ensink NG, Basse PH, Hokland M, Nannmark U, Eggermont AM, et al. The microscopic anatomy of experimental rat CC531 colon tumour metastases: consequences for immunotherapy? Clin Exp Metastasis. 2000;18(2):189-96. PubMed PMID: 11235995.
  2. Hagenaars M, Koelemij R, Ensink NG, van Eendenburg JD, van Vlierberghe RL, Eggermont AM, et al. The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis. 2000;18(4):281-9. PubMed PMID: 11448057.
Storage

Store undiluted at 2-8°C for two months or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Rat.
Specificity
Specificity:
MG1 is a Rat strain-independent markers for tumour cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumour-bearing rats, MG1 localizes to tumour cells (Hagenaars et al., 2001).
This Monoclonal antibody recognizes a cell surface structure of about 80 kD expressed by Rat tumour cells of epithelial origin.
It is suitable for in vivo applications as it has no detectable binding to other cell types then tumour cells when injected in Rats.
MG1 is also useful for immunohistochemical detection of tumour cells.
This Monoclonal antibody clone MG1 belongs to a second generation of antibodies directed against tumours of epithelial origin that were strictly selected for minimal cross reactivity with other tissues.

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