AM32149PU-N Rat Tumors, epithelial origin antibody

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0.1 mg / €810.00
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Mouse anti Rat Rat Tumors, epithelial origin CC52

AM32149PU-N

Product Description for Rat Tumors, epithelial origin

Mouse anti Rat Rat Tumors, epithelial origin CC52.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation, Paraffin Sections, Frozen Sections.

Properties for Rat Tumors, epithelial origin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Rat Tumor Marker
Presentation Purified
Reactivity Rt
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone CC52
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Immunization of mice with Rat colon adenocarcinoma cells (CC531).
Remarks:
Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001).
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application

ELISA. 
Western blotting. 
Flow Cytometry.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Tissue:
There is some cross reactivity with intestinal epithelial tissue and bile ducts. Citric acid pretreatment is recommended.

Background

Common epithelial tumors begin in the surface epithelium of the ovaries and account for about 90% of all ovarian cancers. They are divided into a number of subtypes - including serous, endometrioid, mucinous, and clear cell tumors - that can be further classified as benign (noncancerous) or malignant (cancerous) tumors.

General Readings
  1. Hagenaars M, Ensink NG, Basse PH, Hokland M, Nannmark U, Eggermont AM, et al. The microscopic anatomy of experimental rat CC531 colon tumour metastases: consequences for immunotherapy? Clin Exp Metastasis. 2000;18(2):189-96. PubMed PMID: 11235995.
  2. Hagenaars M, Koelemij R, Ensink NG, van Eendenburg JD, van Vlierberghe RL, Eggermont AM, et al. The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis. 2000;18(4):281-9. PubMed PMID: 11448057.
Storage

Store undiluted at 2-8°C for Two months or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Rat.
Specificity
Specificity:
CC52 is a Rat strain-independent marker for tumour cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumour-bearing rats, CC52 localized to tumour cells (Hagenaars et al., 2001). 
CC52 has been used in many studies in a rat model of colorectal cancer (Hagenaars et al., 2000).
This Monoclonal antibody clone CC52 belongs to a first generation of such antibodies and is suitable to detect tumour cells by Immunohistochemistry. 
It binds to a dimer of two proteins, 120 kD and 130 kD, expressed by Rat tumour cells of epithelial origin. 
The antigens detected by CC52 are also expressed by IL-2-activated cultured NK cells and T cells and by bone marrow cells.

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