MAB5199 Rat Tumors, epithelial origin antibody

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Mouse anti Rat Rat Tumors, epithelial origin CC52


Product Description for Rat Tumors, epithelial origin

Mouse anti Rat Rat Tumors, epithelial origin CC52.
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Rat Tumors, epithelial origin

Product Category Primary Antibodies
Quantity 0.1 mg
Reactivity Rt
Applications C, WB
Clonality Monoclonal
Clone CC52
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer Abnova Taiwan Corp.
Manufacturer certificates ISO 13485:2003

Datasheet Extract

Rat colon adenocarcinoma cells (CC531).
Gene Accession:
Add. information Mouse monoclonal antibody raised against Rat Tumors, epithelial origin.
Application IHC,WB
Storage Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Citric acid pretreatment is recommended for formalin-fixed/paraffin-embedded tissue sections
Buffer System:
(0.05% sodium azide)
CC52 is a rat strain-independent marker for tumor cells of epithelial origin, such as colon, breast, or lung cancer, etc. When injected in colon tumor-bearing rats, CC52 localized to tumor cells (Hagenaars et al., 2001). CC52 can be used for immunohistochemical staining of tumor cells in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. CC52 is also suitable for flow cytometry, Western blotting, immunoprecipitation, and ELISA. Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001). CC52 belongs to a first generation of such antibodies and is suitable to detect tumor cells by immunohistochemistry. The monoclonal antibody binds to a dimer of two proteins, 120 kD, 130 kD, expressed by rat tumor cells of epithelial origin. CC52 has been used in many studies in a rat model of colorectal cancer (Hagenaars et al., 2000). In immunohistochemical stainings, there is some cross reactivity with intestinal epithelial tissue, bile ducts.

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