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DM3239 Renal Cell Carcinoma Marker (gp200) antibody

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Mouse anti Human, Rat Renal Cell Carcinoma Marker (gp200) B512 (PN-15)

DM3239

Product Description for Renal Cell Carcinoma Marker (gp200)

Mouse anti Human, Rat Renal Cell Carcinoma Marker (gp200) B512 (PN-15).
Presentation: Supernatant
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Renal Cell Carcinoma Marker (gp200)

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms Proximal Nephrogenic Renal Antigen
Presentation Supernatant
Reactivity Hu, Rt
Applications P, WB
Clonality Monoclonal
Clone B512 (PN-15)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Microsomal fraction of human renal cortical tissue homogenate
Application Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections (1:50-1:100; Antigen demasking by protease treatment is required. Cellular Localization: Cell membrane, Cytoplasmic. Recommended Positive Control: Normal kidney or renal cell carcinoma). Western Blotting (1:100-1:200, reduced and non-reduced, see ref. #1). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.25 mg/ml IgG
General Readings
  1. Yoshida, S.O etal, Can
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Supernatant
Specificity
Specificity:
This antibody reacts with a 200kD glycoprotein (gp200), which is also known as PNRA (Proximal Nephrogenic Renal Antigen). The antigen is a carbohydrate domain. Reacts with Human and Rat. Others not tested.
In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty-one other normal tissues do not express similar or cross-reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.

Mol. Wt of Antigen: ~200kD

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

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