NB100-75343 Replication factor C subunit 3 antibody

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Chicken anti Human, Rat Replication factor C subunit 3


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  • NB100-75343

Product Description for Replication factor C subunit 3

Chicken anti Human, Rat Replication factor C subunit 3.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Replication factor C subunit 3

Product Category Primary Antibodies
Quantity 0.05 mg
Synonyms activator 1
Presentation Aff - Purified
Reactivity Hu, Rt
Applications E, WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The immunogen for this antibody was a sequence from 1a.a. to 356a.a. from Replication factor C subunit 3(a 356a.a. long protein)
Add. information Research Areas: Stem Cell Markers
Application ELISA, Western Blot, ELISA: Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/ml) and serial dilutions of antibody., ELISA Western Blot
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
General Readings
  1. Kimura,K., Wakamatsu,A., Suzuki,Y., Ota,T., Nishikawa,T., Yamashita,R., Yamamoto,J., Sekine,M., Tsuritani,K., Wakaguri,H.,, et al. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes
  2. Zou,L., Liu,D. and Elledge,S.J., et al. Replication protein A-mediated recruitment and activation of Rad17 complexes
  3. Ohta,S., Shiomi,Y., Sugimoto,K., Obuse,C. and Tsurimoto,T., et al. A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein
  4. Zhang,G., Gibbs,E., Kelman,Z., O'Donnell,M. and Hurwitz,J., et al. Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen
  5. Ellison,V. and Stillman,B., et al. Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity
  6. , O'Donnell M., Onrust R., Dean F.B., Chen M., Hurwitz J. Homology in accessory proteins of replicative polymerases -- E. coli to humans.
  7. , Rieder M.J., Braun A.C., Montoya M.A., Chung M.-W., Nguyen C.P., Nguyen D.A., Livingston R.J., Poel C.L., Robertson P.D., Schackwitz W.S., et al. NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
  8. , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
  9. , Rauen M., Burtelow M.A., Dufault V.M., Karnitz L.M. The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.
  10. , Lindsey-Boltz L.A., Bermudez V.P., Hurwitz J., Sancar A. Purification and characterization of human DNA damage checkpoint Rad complexes.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
No Preservative
Immunogen affinity purified
Aff - Purified
Replication factor C subunit 3
Human, Rat

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