AM05895PU-N Rhizopus Arrhizus antibody

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0.25 mg / €550.00
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Mouse anti Rhizopus Arrhizus WSSA-RA-1

Product Description for Rhizopus Arrhizus

Mouse anti Rhizopus Arrhizus WSSA-RA-1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for Rhizopus Arrhizus

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Applications E, P, WB
Clonality Monoclonal
Clone WSSA-RA-1
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
Isotype control SM13P
Application Immunohistochemistry on paraffin sections: 1:50 (see ref. 1).
Western blot: detects diffuse band/s of approximately 14-110kDa of Rhizopus arrhizus water-soluble somatic antigens.
Does not work in Immunoprecipitation.
Background R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.

Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
Concentration 1.0 mg/ml
General Readings
  1. Jensen HE, Aalbaek B, Lind P, Krogh HV. Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies. Vet Pathol. 1996 Mar;33(2):176-83. PubMed PMID: 8801711.
  2. Jensen HE, Schønheyder HC, Hotchi M, Kaufman L. Diagnosis of systemic mycoses by specific immunohistochemical tests. APMIS. 1996 Apr;104(4):241-58. PubMed PMID: 8645463.
  3. Jensen HE, Salonen J, Ekfors TO. The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies. J Pathol. 1997 Jan;181(1):100-5. PubMed PMID: 9072010.
  4. Jensen HE, Halbaek B, Lind P, Krogh HV, Frandsen PL. Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis. J Vet Diagn Invest. 1996 Jan;8(1):68-75. PubMed PMID: 9026084.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Affinity chromatography
Buffer System:
Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3)
Purified IgM prepared by affinity chromatography from tissue culture supernatant
Liquid Ig fraction
This antibody specifically recognises Rhizopus arrhizus and other members of the family Mucoraceae, known as Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present.

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