AM08457D8-N Rhodamine antibody

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Mouse anti Rhodamine 11H10

Product Description for Rhodamine

Mouse anti Rhodamine 11H10.
Presentation: IRDYE800
Product is tested for Western blot / Immunoblot.

Properties for Rhodamine

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TAMRA, TRITC, Tetramethyl Rhodamine Isothiocyanate
Presentation IRDYE800
Applications WB
Clonality Monoclonal
Clone 11H10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Rhodamine conjugated KLH
Application Western blot: 1:10,000 to 1:25,000 is suggested to detect 12-25 pg of immobilized protein.
Immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Conjugation: LI-COR Biosciences, Lincoln, NE.
Storage Store vial at 2-8° C prior to restoration. Centrifuge
product if not completely clear after standing at room temperature. This product is stable at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
Shelf life: One year from despatch.
IRDy(TM)800 (MW 1166.2)
Absorption emission:
774 nm (in PBS) / 800 nm
Molar radio:
3.0 moles IRDy(TM)800/mole of Mouse IgG
Protein A chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA IgG and Protease free, 0.01% (w/v) Sodium Azide
Lyophilized Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This antibody reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.

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