TA309469 Rhodopsin (N-term) antibody

Mouse Monoclonal anti-RHO Antibody

See related secondary antibodies

Search for all "Rhodopsin"

0.1 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Amphibian, Bovine, Fish, Frog, Human, Mammalia, Mouse, Porcine, Rat, Zebrafish Rhodopsin 4D2

Product Description for Rhodopsin

Mouse anti Amphibian, Bovine, Fish, Frog, Human, Mammalia, Mouse, Porcine, Rat, Zebrafish Rhodopsin 4D2.
Properties: (N-term)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Rhodopsin

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms OPN2, Opsin-2, RHO
Presentation Purified
Reactivity Amph, Bov, Fi, Frog, Hu, Mam, Ms, Por, Rt, Ze
Applications C, E, ICC/IF, IP, WB
Clonality Monoclonal
Clone 4D2
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P08100
Immunogen:
Bovine Rhodopsin.
GeneID:
6010
Property N-term
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA.
Western blot: 1/1000 (1)
1 µg/ml was sufficient for detection of Rhodopsin in 10 µg of Rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Immunoprecipitation: 1–2 µg per 100–500 µg of protein
Immunohistochemistry.
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Concentration 1.0 mg/ml
General Readings
  1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
  2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
  3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
  4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
  5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography.
Buffer System:
PBS pH 7.4 containing 50% Glycerol and 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Species Reactivity
Species reactivity (tested):
All Mammalian species (Human, Mouse, Rat, Bovine, Pig, etc), Most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
Specificity
Specificity:
N-terminus of Rhodopsin.

Accessory Products

Proteins and/or Positive Controls

Positive controls for Rhodopsin (1 products)

Catalog No. Species Pres. Purity   Source  

RHO overexpression lysate

RHO overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn