TA309470 Rhodopsin (C-term) antibody

Mouse Monoclonal anti-RHO Antibody

See related secondary antibodies

Search for all "Rhodopsin"

0.1 mg / €410.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Vertebrates Rhodopsin 1D4

Product Description for Rhodopsin

Mouse anti Vertebrates Rhodopsin 1D4.
Properties: (C-term)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Rhodopsin

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms OPN2, Opsin-2, RHO
Presentation Purified
Reactivity Ve, Vertebrates.
Applications C, E, ICC/IF, IP, WB
Clonality Monoclonal
Clone 1D4
Host Mouse
Isotype IgG1
Molecular weight 48 kDa
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P08100
Immunogen:
Bovine Rhodopsin.
GeneID:
6010
Property C-term
Isotype control AM03095PU-N
Application ELISA.
Western blot: 1/1000 (Ref.1)
1 µg/ml was sufficient for detection of Rhodopsin in 10 µg of Rat eye lysate by colorimetric immunoblot analysis using Goat anti-Mouse IgG:HRP as the secondary antibody. 
Immunofluorescence.
Immunoprecipitation: 1–2 µg per 100–500 µg of protein.
Immunohistochemistry.
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (Ref.2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Cheng CL, Djajadi H, Molday RS. Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy. Methods Mol Biol. 2013;935:185-99. doi: 10.1007/978-1-62703-080-9_12. PubMed PMID: 23150368.
General Readings
  1. Molday RS, Hicks D, Molday L. Peripherin. A rim-specific membrane protein of rod outer segment discs. Invest Ophthalmol Vis Sci. 1987 Jan;28(1):50-61. PubMed PMID: 2433249.
  2. Ridge KD, Lee SS, Abdulaev NG. Examining rhodopsin folding and assembly through expression of polypeptide fragments. J Biol Chem. 1996 Mar 29;271(13):7860-7. PubMed PMID: 8631831.
  3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
  4. Feuerstein SE, Pulvermüller A, Hartmann R, Granzin J, Stoldt M, Henklein P, et al. Helix formation in arrestin accompanies recognition of photoactivated rhodopsin. Biochemistry. 2009 Nov 17;48(45):10733-42. doi: 10.1021/bi900544p. PubMed PMID: 19835414.
  5. Iannaccone A, Man D, Waseem N, Jennings BJ, Ganapathiraju M, Gallaher K, et al. Retinitis pigmentosa associated with rhodopsin mutations: Correlation between phenotypic variability and molecular effects. Vision Res. 2006 Dec;46(27):4556-67. Epub 2006 Oct 2. PubMed PMID: 17014888.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
50% Glycerol
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Vertebrates.
Specificity
Specificity:
Recognizes Rhodopsin (~40kDa).
Reacts with both native and recombinant protein. No known reactivity to other proteins.
The antibody specifically binds to the C-terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of Rhodopsin.
Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Accessory Products

Proteins and/or Positive Controls

Positive controls for Rhodopsin (1 products)

Catalog No. Species Pres. Purity   Source  

RHO overexpression lysate

RHO overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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