AM32070PU-N Ribonucleoprotein (RNP) antibody

See related secondary antibodies

Search for all "Ribonucleoprotein (RNP)"

0.1 mg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Ribonucleoprotein (RNP) 58-15

AM32070PU-N

Product Description for Ribonucleoprotein (RNP)

Mouse anti Human Ribonucleoprotein (RNP) 58-15.
Presentation: Purified
Product is tested for Flow Cytometry, Paraffin Sections, Frozen Sections.

Properties for Ribonucleoprotein (RNP)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone 58-15
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Isolated nuclei.
Splenocytes were fused with mouse myeloma NS-1 cells.
Isotype control SM13P
Application Flow Cytometry: The cells have to be made permeable and incubation with 58-15 antibody should be carried out at 37°C.
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections.
Background Anti-nuclear antibodies remain prevalent in a large group of autoimmune disorders. The accumulation of anti-nuclear antibodies is characteristic of lupus erythematosus, as well as various other auto-immune diseases such as Sjögren's syndrome, autoimmune hepatitis, dermatomyositis, rheumatoid arthritis, and scleroderma. Ribonucleoproteins (RNP) represent a 20-80nm electron dense nuclear structure, with highest labeling densities  found in nuclear ribonucleoprotein (nRNP) particles. One of the main components of the nucleolus,  RNPs are comprised of ribonucleic acid (RNA) and protein together, representing an RNA binding motif in an RNA binding protein. Aromatic amino acid residues occupying this RNP motif, create stacking interactions with RNA. Lysine residues expressed exclusively in the helical portion of RNA binding proteins stabilizes relationships with nucleic acids. 
The ability to quantitate and identify dividing cells facilitates immunopathologic studies on tissues from which prognostic information can be derived for disease states such as cancer.
Concentration 0.1 mg/ml
General Readings
  1. SWIFT H. CYTOCHEMICAL STUDIES ON NUCLEAR FINE STRUCTURE. Exp Cell Res. 1963;24:SUPPL9:54-67. PubMed PMID: 14046254.
  2. Clevenger CV, Epstein AL. Use of immunogold electron microscopy and monoclonal antibodies in the identification of nuclear substructures. J Histochem Cytochem. 1984 Jul;32(7):757-65. PubMed PMID: 6376619.
  3. Clevenger CV, Bauer KD, Epstein AL. A method for simultaneous nuclear immunofluorescence and DNA content quantitation using monoclonal antibodies and flow cytometry. Cytometry. 1985 May;6(3):208-14. PubMed PMID: 3888556.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal antibody 58-15 recognizes Nuclear Ribonucleoprotein particles in Human cells.
58-15 identifies cells active in the cell cycle and hence can be used to measure the mitotic activity of cell populations. 
Since the antibody can be used in Paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens.

Accessory Products

  • LinkedIn