NB100-662 Ribosomal RNA (rRNA 5.8s) antibody

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Mouse anti All Species Ribosomal RNA Y10b

Product Description for Ribosomal RNA

Mouse anti All Species Ribosomal RNA Y10b.
Properties: (rRNA 5.8s)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Ribosomal RNA

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Purified
Reactivity All Species
Applications ICC/IF, IP
Clonality Monoclonal
Clone Y10b
Host Mouse
Isotype IgG3
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The whole 5.8S ribosomal RNA (see reference 11).
Property rRNA 5.8s
Isotype control AM03097PU-N
Application Immunocytochemistry, immunofluorescence, immunoprecipitation, The investigator should determine the optimal working dilution for a specific application., immunofluorescence , immunoprecipitation 10 ul/lysate, Immunocytochemistry
Background The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro. This antibody is useful for recognizing RNA epitopes on both the large and small subunit.
Concentration 1 mg/ml
General Readings
  1. Aakalu, G., Smith, W.B., Nguyen, N., Jiang, C., and Schuman, E.M. Neuron. 30:489-502, 2001.
  2. Anderson, K.D., Merhege, M.A., Morin, M., Bolognani, F., and Perrone-Bizzozero, N.I. Experimental Neurology. 183:100-108, 2003.
  3. Bleher, R. and Martin, R. Neuroscience. 107:527-534, 2001.
  4. Fan, X.C. and Steitz, J.A. EMBO Journal. 17:3448-3460, 1998.
  5. Gallouzi, I-E., Brennan, C.M., Stenberg, M.G., Swanson, M.S., Eversole, A., Maizels, N., and Steitz, J.A. PNAS USA. 97:3073-3078, 2000. (Human, Immunofluorescence)
  6. Jablonka, S., Wiese, S., and Sendtner, M. Journal of Neurobiology. 58:272-286, 2004.
  7. Sotelo-Silveira, J.R., Calliari, A., Cárdenas, M., Koenig, E., and Sotelo, J.R. Journal of Neurobiology. 187-196, 2004.
  8. Stone, J.S., and Rubel, E.W. Journal of Comparative Neurology. 417:1-16, 2000.
  9. Thomas, M.G., Martinez Tosar, L.J., Loschi, M., Pasquini, J.M., Correale, J., Kindler, S., and Boccaccio, G.L. Molecular Biology of the Cell. 16:405-420, 2005.
  10. Zheng, J., Kelly, T.K., Chang, B., Ryazantsev, S., Rajasekaran, A.K., Martin, K.C., and Twiss, J.L. Journal of Neuroscience, 21: 9291-930, 2001.
  11. Lerner, E.A., Lerner, M.R., Janeway, C.A. and Steitz, J.A. PNAS USA. 78(5):2737-2741, 1981. (Mouse, Immunoprecipitation)
  12. Abou, E.S. & Nazar, R.N. Role of the 5.8S rRNA in ribosome translocation. Nucleic Acid Res. 25(9):1788-1794, 1997.
  13. Garden, G.A., et al. Protein masking of a ribosomal RNA epitope is an early event in afferent deprivation-induced neuronal death. Molecular & Cell. Neurosci. 6:293-310, 1995. (Chicken, Immunohistochemistry)
Storage Store at 4C. Do not freeze.
protein A purified
Buffer System:
0.05% Sodium Azide
protein A purified
This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
This antibody reacts with all eukaryotes.

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