AM05879PU-N Rituximab antibody

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0.2 mg / €910.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Rituximab MB2 A4

Product Description for Rituximab

Rat anti Rituximab MB2 A4.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.

Properties for Rituximab

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms MabThera, Rituxan
Presentation Purified
Applications E, F
Clonality Monoclonal
Clone MB2 A4
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
F(ab)2 fragment of Rituximab.
Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line
Application ELISA: 5 µg/ml as coating antibody.
Flow Cytometry: Use 10 µl of 50 µg/ml diluted antibody to label 106 cells in 100 µl.
Background Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
Concentration 1.0 mg/ml
General Readings 1. Bayne, M. et al. (2003) Phase I/II study of fractionated radioimmunotherapy in relapsed low grade non Hodgkin lymphoma. Br. J. Cancer. 88 : S38.
2. Cragg, M. S. et al. (2004) Apparent modulation of CD20 by rituximab: an alternative explanation.
Blood. 103: 3989-3990. 3. Beum, P. V. et al. (2004) Complement activation and C3b deposition on rituximab-opsonized cells substantially blocks binding of phycoerythrin-labeled anti-mouse IgG probes to rituximab. J. Immunol. Methods. 294: 37-42.
4. Hampson, G. et al. (2010) Validation of an ELISA for the determination of rituximab pharmacokinetics in clinical trials subjects. J. Immunol. Methods 360: 30-38.
5. Blasco, H. et al. (2007) Evaluation of a peptide ELISA for the detection of rituximab in serum. J Immunol Methods.325: 127-39.
6. Daydé, D. et al. (2009) Tumor burden influences exposure and response to rituximab: pharmacokinetic-pharmacodynamic modeling using a syngeneic bioluminescent murine model expressing human CD20. Blood. 113: 3765-72.
7. Aung, T. et al. (2011) Exosomal evasion of humoral immunotherapy in aggressive B-cell lymphoma modulated by ATP-binding cassette transporter A3. Proc Natl Acad Sci U S A. 108: 15336-41.
8. Schmidt, E. et al. (2009) Immunogenicity of rituximab in patients with severe pemphigus. Clin Immunol. 132: 334-41.
9. McDonald, V. et al. (2010) Rituximab pharmacokinetics during the management of acute idiopathic thrombotic thrombocytopenic purpura. J Thromb Haemost. 8: 1201-8.
10. Kagan, L. et al. (2011) Subcutaneous Absorption of Monoclonal Antibodies: Role of Dose, Site of Injection, and Injection Volume on Rituximab Pharmacokinetics in Rats. Pharm Res. Sep 2. [Epub ahead of print].
11. Kagan, L. and Mager, D.E. (2013) Mechanisms of subcutaneous absorption of rituximab in rats. Drug Metab Dispos. 41: 248-55.
12. Cragg, M. S. et al. (2004) A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells. Blood. 104:2540-2.
13. Kagan, L. et al. (2014) Interspecies pharmacokinetic modeling of subcutaneous absorption of rituximab in mice and rats. Pharm Res. 31: 3265-73.
14. Blasco, H. et al. (2009) Pharmacokinetics of rituximab associated with CHOP chemotherapy in B-cell non-Hodgkin lymphoma. Fundam Clin Pharmacol. 23: 601-8.
15. Pers, J.O. et al. (2007) BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome. Arthritis Rheum. 56: 1464-77.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
TRIS buffered saline pH 8.0
Preservatives:
0.09% Sodium Azide
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Specificity
Specificity:
This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. It does not recognise other CD20 antibodies.
This antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy.
It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).

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