NB100-63055 ROOTLETIN antibody

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Human anti Mouse ROOTLETIN 2085

Product Description for ROOTLETIN

Human anti Mouse ROOTLETIN 2085.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for ROOTLETIN

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Ms
Applications C, E, P, WB
Clonality Monoclonal
Clone 2085
Host Human
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

A fusion protein of a domain of murine mKIAA0445 (amino acid residues 4855-5352, MW 19 kDa) fused expressed in E. coli.
Add. information Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. HuCal and HuCal Gold are registered trademarks of MorphoSys.
Application ELISA, Immunohistochemistry-Frozen, Western Blot, This antibody is useful for ELISA, Immunohistochemistry-Frozen, Western Blot, ELISA 2ug/ml, Western Blot 2ug/ml, Immunohistochemistry-Frozen 3.4ug/ml
Concentration 1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Affinity purified
Buffer System:
No Preservative
Affinity purified
Aff - Purified
NB100-63055 binds to the C-terminal 166 amino acids of mKIAA0445 protein, a fragment of rootletin, the structural component of the ciliary rootlets. The ciliary rootlet, first recognized over a century ago, is a prominent structure originating from ththe basal body at the proximal end of a cilium. Despite being the largest cytoskeleton, its structural composition has long remained unknown. Rootletin is a 220 kDa protein, found in the rootlets of ciliated cells. Recombinant rootletin forms detergent-insoluble filaments radiating from the centrioles and resembling rootlets found in vivo. Rootletin has a globular head domain and a tail domain con-sisting of extended coiled-coil structures. Rootletin forms parallel in register homodimers and elongated higher order polymers mediated by the tail domain alone. The head domain may be required for targeting to the basal body and binding to a kinesin light chain. In retinal photoreceptors where rootlets appear particularly robust, rootlets extend from the basal bodies to the synaptic terminals and anchor ER membranes along their length. Our data indicate that rootlets are composed of homopolymeric rootletin protofilaments bundled into variably shaped thick filaments. NB100-63055 may bind to human and rat rootletin, due to 89% and 88% sequence identity in the antigen region

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