Product Description for RPS6KB1 / STK14A
Rabbit anti Human, Mouse, Rat RPS6KB1 / STK14A.
Properties: pThr444
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Properties: pThr444
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Properties for RPS6KB1 / STK14A
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 mg |
| Synonyms | 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1 |
| Presentation | Aff - Purified |
| Reactivity | Hu, Ms, Rt |
| Applications | ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Molecular weight | ~59,70,85 kDa |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | pThr444 |
| Application | Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200. |
| Background | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal.Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region. Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression. Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase. |
| Concentration | 1.0 mg/ml |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 6198 |
Accessory Products
Proteins and/or Positive Controls
Proteins for RPS6KB1 / STK14A (14 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP317324 |
RPS6KB1 / STK14A |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00006198-P01-10 |
RPS6KB1 / STK14A |
||||||
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue | ||||
| Abnova Taiwan Corp. | |||||||
| H00006198-P01-25 |
RPS6KB1 / STK14A |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue | ||||
| Abnova Taiwan Corp. | |||||||
| H00006198-Q01-10 |
RPS6KB1 / STK14A |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00006198-Q01-25 |
RPS6KB1 / STK14A |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P1441 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1442 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1443 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1444 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1445 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P1447 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4209 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P4772 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5755 |
RPS6KB1 / STK14A |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for RPS6KB1 / STK14A (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00006198-T01 |
RPS6KB1 293T Cell Transient Overexpression Lysate(Denatured) |
||||||
|
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| Abnova Taiwan Corp. | |||||||
| LY401097 |
RPS6KB1 overexpression lysate |
||||||
|
0.1 mg /
€495.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| NBL1-15574 |
S6K Lysate |
||||||
![Western Blot: S6K Lysate [NBL1-15574] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for RPS6KB1](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/S6K%20Lysate-Western%20Blot-NBL1-15574-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for RPS6KB1 / STK14A (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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