discontinued

AM10218SU-N Rubella virus glycoprotein E1 antibody

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0.2 ml / €400.00

Quick Overview

Mouse anti Rubella virus glycoprotein E1 5D11

AM10218SU-N

Product Description for Rubella virus glycoprotein E1

Mouse anti Rubella virus glycoprotein E1 5D11.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Rubella virus glycoprotein E1

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Ascites
Applications C, E, WB
Clonality Monoclonal
Clone 5D11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Recombinant E1H Rubella virus
Application ELISA : 1/2000-1/10000. 
Western Blot: 1/200-1/1000. 
Immunocytochemistry on infected cells (IF): 1/100-1/500.
Background Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.
Storage

Prior to reconstitution store the antibody at -20°C.
Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Preservatives:
None
State:
Lyophilized powder
Ascites
Reconstitution:
Restore in distilled water.
Specificity
Specificity:
Reacts with E1 hemagglutinin antigen, specific for Rubella virus (RUBV).
No cross reaction with other viruses.

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