AP55801PU-S RUNX1 pSer435 antibody

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50 µg / €270.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat RUNX1

Product Description for RUNX1

Rabbit anti Human, Mouse, Rat RUNX1.
Properties: pSer435
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for RUNX1

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms 1, AML-1, AML1, AML1-EVI-1, AMLCR1, Acute myeloid leukemia 1 protein, CBF-alpha-2, CBFA2, Core-binding factor subunit alpha-2, EVI-1, PEA2-alpha B, PEBP2-alpha B, PEBP2A2, Polyomavirus enhancer-binding protein 2 alpha B subunit, Runt-related transcription factor, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Molecular weight 53 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q01196
Immunogen:
Peptide sequence around phosphorylation site of Serine 435( S-N-S(p)-P-T) derived from Human AML1 (KLH-conjugated)
GeneID:
861
Property pSer435
Application Western blot: 1:500~1:1000.
Background CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Concentration 1.0 mg/ml
General Readings
  1. Miyoshi H, Shimizu K, Kozu T, Maseki N, Kaneko Y, Ohki M. t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10431-4. PubMed PMID: 1720541. (Free PMC Article available)
  2. Sacchi N, Nisson PE, Watkins PC, Faustinella F, Wijsman J, Hagemeijer A. AML1 fusion transcripts in t(3;21) positive leukemia: evidence of molecular heterogeneity and usage of splicing sites frequently involved in the generation of normal AML1 transcripts. Genes Chromosomes Cancer. 1994 Dec;11(4):226-36. PubMed PMID: 7533526.
  3. Nucifora G, Birn DJ, Espinosa R, Erickson P, LeBeau MM, Roulston D, et al. Involvement of the AML1 gene in the t(3;21) in therapy-related leukemia and in chronic myeloid leukemia in blast crisis. Blood. 1993 May 15;81(10):2728-34. PubMed PMID: 8490181.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography using epitope-specific peptide
Buffer System:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat
Specificity
Specificity:
The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

Accessory Products

Proteins and/or Positive Controls

Proteins for RUNX1 (3 products)

Catalog No. Species Pres. Purity   Source  

RUNX1 (transcript variant 2)

RUNX1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RUNX1

RUNX1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

RUNX1

RUNX1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for RUNX1 (3 products)

Catalog No. Species Pres. Purity   Source  

RUNX1 Lysate

Western Blot: RUNX1 Lysate [NBL1-15624] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for RUNX1
  Novus Biologicals Inc.

RUNX1 overexpression lysate

RUNX1 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

RUNX1 overexpression lysate

RUNX1 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
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