SM5119 Ryanodine receptor 1 / RYR1 antibody

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0.1 ml / €470.00

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Mouse anti Amphibian, Bovine, Canine, Fish, Human, Mouse, Rabbit, Rat, Sheep, Mustelid Ryanodine receptor 1 / RYR1 34C

Product Description for Ryanodine receptor 1 / RYR1

Mouse anti Amphibian, Bovine, Canine, Fish, Human, Mouse, Rabbit, Rat, Sheep, Mustelid Ryanodine receptor 1 / RYR1 34C.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Ryanodine receptor 1 / RYR1

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms RYDR, RYR-1
Presentation Purified
Reactivity Amph, Bov, Can, Fi, Hu, Md, Ms, Rb, Rt, Sh
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone 34C
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Partially purified Chicken pectoral muscle Ryanodine Receptor
Application Immunofluorescence.
Immunohistochemistry on Frozen Sections:
Results in intense staining of cerebellar Purkinje neurons in Chicken brain.
Western Blot: 1/5000. Detects a 565 kDa protein representing RyR from Rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.
Background The ryanodine receptor (RyR) is the channel responsible for calcium release from muscle cell sarcoplasmic reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR.
Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
Concentration 1.0 mg/ml
General Readings
  1. Yusufi AN, Cheng J, Thompson MA, Dousa TP, Warner GM, Walker HJ, et al. cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells. Am J Physiol Renal Physiol. 2001 Jul;281(1):F91-F102. PubMed PMID: 11399650.
  2. Airey JA, Grinsell MM, Jones LR, Sutko JL, Witcher D. Three ryanodine receptor isoforms exist in avian striated muscles. Biochemistry. 1993 Jun 8;32(22):5739-45. PubMed PMID: 7684927.
  3. Biophysical Journal, 59: 1153-1163, 1995.
  4. Liu G, Pessah IN. Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls. J Biol Chem. 1994 Dec 30;269(52):33028-34. PubMed PMID: 7806531.
  5. Walton PD, Airey JA, Sutko JL, Beck CF, Mignery GA, Südhof TC, et al. Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons. J Cell Biol. 1991 Jun;113(5):1145-57. PubMed PMID: 1645737. (Free PMC Article available)
  6. Chevessier F, Bauché-Godard S, Leroy JP, Koenig J, Paturneau-Jouas M, Eymard B, et al. The origin of tubular aggregates in human myopathies. J Pathol. 2005 Nov;207(3):313-23. PubMed PMID: 16178054.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A Chromatography
0.05% Sodium Azide
Liquid purified IgG fraction
This antibody detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms in Amphibian, Bovine, Canine, Fish, Human, Non-Human Primate, Mouse, Rabbit, Mink, Rat and Sheep tissues.
In chickens this antibody detects the alpha, beta and cardiac isoforms.
This antibody also detects RyR-3 in Mouse cells. In Frog, SM5119 detects the alpha and beta isoforms and in Fish it detects the alpha isoform.
Human, Mouse, Rat, Canine, Rabbit, Non-Human Primate, Bovine, Ovine, Amphibian, Fish and Mustelid.

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