SM1852 Salmonid Ig antibody

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0.5 ml / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Salmonid Salmonid Ig 5F12

SM1852

Product Description for Salmonid Ig

Mouse anti Salmonid Salmonid Ig 5F12.
Presentation: Supernatant
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Salmonid Ig

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms Salmon Ig, Salmon immunoglobulin
Presentation Supernatant
Reactivity Salmonid
Applications E, ICC/IF, WB
Clonality Monoclonal
Clone 5F12
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Rainbow trout and Atlantic salmon immunoglobulins.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NSO myeloma cell line.
Application ELISA: 1/250-1/1000.
Western blot: 1/250-1/1000. This antibody detects a band of app. 71 kDa using Salmon ummune serum.
Immunofluorescence.
Flow Cytometry.
Does NOT work on Immunohistochemistry sections.
General Readings
  1. Bakke-McKellep AM, Sanden M, Danieli A, Acierno R, Hemre GI, Maffia M, et al. Atlantic salmon (Salmo salar L.) parr fed genetically modified soybeans and maize: Histological, digestive, metabolic, and immunological investigations. Res Vet Sci. 2008 Jun;84(3):395-408. PubMed PMID: 18561390.
  2. Raida MK, Nylén J, Holten-Andersen L, Buchmann K. Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri. PLoS One. 2011;6(6):e18832. doi: 10.1371/journal.pone.0018832. Epub 2011 Jun 24. PubMed PMID: 21731605. (Free PMC Article available, 5 images available)
  3. von Gersdorff Jørgensen L, Heinecke RD, Skjødt K, Rasmussen KJ, Buchmann K. Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis. 2011 Oct;34(10):749-55. doi: 10.1111/j.1365-2761.2011.01291.x. PubMed PMID: 21916900.
  4. Rømer Villumsen K, Dalsgaard I, Holten-Andersen L, Raida MK. Potential role of specific antibodies as important vaccine induced protective mechanism against Aeromonas salmonicida in rainbow trout. PLoS One. 2012;7(10):e46733. doi: 10.1371/journal.pone.0046733. Epub 2012 Oct 8. PubMed PMID: 23056427. (Free PMC Article available, 4 images available)
  5. Skov, J. et al. (2012) Immunomodulatory effects of dietary ß-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri. Fish Shellfish Immunol. 33: 111-20.
  6. Deshmukh S, Kania PW, Chettri JK, Skov J, Bojesen AM, Dalsgaard I, et al. Insight from molecular, pathological, and immunohistochemical studies on cellular and humoral mechanisms responsible for vaccine-induced protection of rainbow trout against Yersinia ruckeri. Clin Vaccine Immunol. 2013 Oct;20(10):1623-41. doi: 10.1128/CVI.00404-13. Epub 2013 Aug 21. PubMed PMID: 23966555. (Free PMC Article available, 9 images available)
  7. Chettri JK, Deshmukh S, Holten-Andersen L, Jafaar RM, Dalsgaard I, Buchmann K. Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections. Vet Immunol Immunopathol. 2013 Jul 15;154(1-2):42-7. doi: 10.1016/j.vetimm.2013.04.001. Epub 2013 Apr 8. PubMed PMID: 23622778.
  8. von Gersdorff Jørgensen L, Sigh J, Kania PW, Holten-Andersen L, Buchmann K, Clark T, et al. Approaches towards DNA vaccination against a skin ciliate parasite in fish. PLoS One. 2012;7(11):e48129. doi: 10.1371/journal.pone.0048129. Epub 2012 Nov 7. PubMed PMID: 23144852. (Free PMC Article available, 9 images available)
  9. Holten-Andersen L, Dalsgaard I, Nylén J, Lorenzen N, Buchmann K. Determining vaccination frequency in farmed rainbow trout using Vibrio anguillarum O1 specific serum antibody measurements. PLoS One. 2012;7(11):e49672. doi: 10.1371/journal.pone.0049672. Epub 2012 Nov 21. PubMed PMID: 23185402. (Free PMC Article available, 5 images available)
  10. Cook, M et al. (2008) Amoebic gill disease (AGD) vaccine development phase II - Molecular basis of host parasite interactions in amoebic gill disease. Aquafin CRC Project 3.4.4(2)
  11. Villumsen KR, Neumann L, Ohtani M, Strøm HK, Raida MK. Oral and anal vaccination confers full protection against enteric redmouth disease (ERM) in rainbow trout. PLoS One. 2014 Apr 4;9(4):e93845. doi: 10.1371/journal.pone.0093845. eCollection 2014. PubMed PMID: 24705460. (Free PMC Article available, 4 images available)
  12. Villiers E, Baines S, Law AM, Mallows V. Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody. Vet Clin Pathol. 2006 Mar;35(1):55-71. PubMed PMID: 16511792.
  13. Henriksen MM, Kania PW, Buchmann K, Dalsgaard I. Evaluation of the immune response in rainbow trout fry, Oncorhynchus mykiss (Walbaum), after waterborne exposure to Flavobacterium psychrophilum and/or hydrogen peroxide. J Fish Dis. 2015 Jan;38(1):55-66. PubMed PMID: 24547972.
  14. Villumsen KR, Koppang EO, Raida MK. Adverse and long-term protective effects following oil-adjuvanted vaccination against Aeromonas salmonicida in rainbow trout. Fish Shellfish Immunol. 2015 Jan;42(1):193-203. doi: 10.1016/j.fsi.2014.09.024. Epub 2014 Sep 30. PubMed PMID: 25281580.
  15. Soltani M, Shafiei Sh, Yosefi P, Mosavi Sh, Mokhtari A. Effect of Montanide™ IMS 1312 VG adjuvant on efficacy of Yersinia ruckeri vaccine in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol. 2014 Mar;37(1):60-5. doi: 10.1016/j.fsi.2013.12.027. Epub 2014 Jan 18. PubMed PMID: 24444826.
  16. Chettri, J.K. et al. (2015) Booster immersion vaccination using diluted Yersinia ruckeri bacterin confers protection against ERM in rainbow trout Aquaculture 440: 1-5.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Concentrated Tissue Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Salmonid.
Specificity
Specificity:
This antibody recognizes immunoglobulin, binding to Ig of the salmo, oncorhynchus and salvelinus geni.
The antibody may be used to detect increased levels of immunoglobulins in fish serum, which may be indicative of a parasitic or viral infection. 
These include: 
Salmo: Atlantic Salmon, Brown Trout.
Oncorhynchus: Chinook Salmon, Chum Salmon, Coho Salmon, Sockeye Salmon, Rainbow Trout.
Salvelinus: Arctic Char, Brook Trout.

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