AP13035PU-N SARS virus PUP1 (N-term) antibody

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Rabbit anti SARS virus PUP1


Product Description for SARS virus PUP1

Rabbit anti SARS virus PUP1.
Properties: (N-term)
Presentation: Purified

Properties for SARS virus PUP1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms SARS-CoV PUP1, Severe acute respiratory syndrome coronavirus Putative Uncharacterized Protein 1
Presentation Purified
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of SARS virus PUP1.
Property N-term
Application ELISA: 1/1,000.
Background The SARS-CoV genome contains five major open reading frames (ORFs) that encode the replicase polyprotein (R), the spike (S), envelope (E), and membrane (M) glycoproteins; and the nucleocapsid protein (N). Other proteins not falling into these categories have been termed PUPs (putative uncharacterized proteins) for their unknown structural or functional features and dissimilarity to those known sequences. However, it has been found that some of the PUPs matched the entries in the NCBI database. PUP1 is equivalent to ORF3 in Isolate Tor2. It receives 11 hits in GenBank through BLAST, two of which are putative transmembrane proteins. One is from Ralstonia solanacearum, cytochrome b-561, with 97 amino acids of PUP1 aligned, and the other is from Sinorhizobium meliloti, with 94 amino acids aligned. Sequence identities are 28% and 25%, respectively. Three putative transmembrane domains are located within PUP1.
Caution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration 0.25 mg/ml
General Readings
  1. He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
  2. Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
  3. Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Buffer System:
PBS with 0.09% (W/V) sodium azide
Liquid purified Ig
This antibody reacts to SARS virus PUP1.

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