AP13044PU-N SARS virus PUP6 (N-term) antibody

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0.1 mg / €335.00

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Rabbit anti SARS virus PUP6


Product Description for SARS virus PUP6

Rabbit anti SARS virus PUP6.
Properties: (N-term)
Presentation: Purified

Properties for SARS virus PUP6

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms SARS-CoV PUP6, Severe acute respiratory syndrome coronavirus Putative Uncharacterized Protein 6
Presentation Purified
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of SARS virus PUP6.
Property N-term
Application ELISA: 1/1,000.
Background An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries. The severity of this disease is such that the mortality rate appears to be ~3 to 6%. A number of laboratories worldwidehave undertaken the identification of the causative agent. The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as SARS virus. The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein. Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.
Concentration 0.25 mg/ml
General Readings
  1. He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
  2. Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
  3. Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Buffer System:
PBS with 0.09% (W/V) sodium azide
Liquid purified Ig
This antibody reacts to SARS virus PUP6.

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