AM26706PU-N SCIMP antibody

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0.1 mg / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human SCIMP NVL-07

AM26706PU-N

Product Description for SCIMP

Mouse anti Human SCIMP NVL-07.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation, Flow Cytometry.

Properties for SCIMP

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms SLP adaptor and Csk interacting membrane protein
Presentation Aff - Purified
Reactivity Hu
Applications F, ICC/IF, IP, WB
Clonality Monoclonal
Clone NVL-07
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6UWF3
Immunogen:
Recombinant intracellular part of human SCIMP
GeneID:
388325
Application Flow cytometry (intracellular staining).
Immunoprecipitation.
Western blot.
Immunocytochemistry.
Background SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.
Concentration 1.0 mg/ml
General Readings
  1. Draber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, et al. SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011 Nov;31(22):4550-62. doi: 10.1128/MCB.05817-11. Epub 2011 Sep 19. PubMed PMID: 21930792. (Free PMC Article available, 8 images available)
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Buffer System:
Phosphate buffered saline (PBS), approx. pH 7.4
Preservatives:
15 mM sodium azide
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
The antibody recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Accessory Products

Proteins and/or Positive Controls

Proteins for SCIMP (1 products)

Catalog No. Species Pres. Purity   Source  

Chromosome 17 open reading frame 87 (C17orf87)

Chromosome 17 open reading frame 87 (C17orf87) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Positive controls for SCIMP (1 products)

Catalog No. Species Pres. Purity   Source  

SCIMP overexpression lysate

SCIMP overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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