discontinued

AP10308PU-N SEC23A antibody

See related secondary antibodies

Search for all "SEC23A"

Quick Overview

Rabbit anti Human SEC23A

Product Description for SEC23A

Rabbit anti Human SEC23A.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for SEC23A

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms COPII, Coat Protein 2, Protein transport protein Sec23A, SEC23-related protein A
Presentation Aff - Purified
Reactivity Hu
Applications IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q15436
Immunogen:
This antibody was generated in rabbits against synthetic peptides from the unique sequence to Sec23 protein.
GeneID:
10484
Application Western Blot: > 1/500.
Immunoprecipitation and i.p pull-down assays: > 1/350.
Background The regulation of intracellular vesicular trafficking is mediated by specific families of proteins that are involved in vesicular budding, translocation, and fusion with target membranes. The protein trafficking between endoplasmic reticulum (ER) and Golgi apparatus is mediated by two distinct membrane coat complexes, COPI and COPII (1). Two Coat protein II (COPII) proteins are necessary to generate secretory vesicles at the endoplasmic reticulum. COPI consist of 7 protein subunits includinga, b, b1, d, e, d, z. COPI is also responsible for the retrograde golgi to ER transport f dilysine-tagged proteins (2). Sec23A is a functional human counter part of the yeast COPII component Sec23p which suggests that it plays similar role in the mammalian protein export from ER. Both Sec 23 isoforms (Sec23A and Sec23B) have similar molecular weight 85 kDa and are co-expressed in many human tissues at varying levels of expression. In yeast, the Sec24p protein is the only COPII component in which two close orthologues have been identified. Sec-23p is a component of coat protein II (COPII) coated vesicles involved in protein export from the endoplasmic reticulum (Mizoguchi et. al., 2000). The COPII is required for the vesicle budding from the endoplasmic reticulum and is consist of two heterodimeric subcomplexes, Sec23p/Sec24p and Sec13p/Sec31p and a small GTPase Ser1p (Higashio et al., 2000). Recent data also suggest that Sec23p/Sec24p complexes are in addition to its role in the shaping up of the vesicle, the Sec23-24p complex may be implicated in cargo selection and trafficking of the amount of cargo with in the cells.
Concentration 1-1.25 mg/ml
General Readings
  1. Weidler, M., Reinhard C., Friedrich G., et. Al., Biochem. Biophys. Res. Comm. 271, 401-408, 2000.
  2. Botelho R. J., Hackam D. J., et. Al., J. Biol. Chem. 275, 10655-10660, 2000.
  3. Mizoguchi T, Nakajima K, Hatsuzawa K, Nagahama M, Hauri HP, Tagaya M, et al. Determination of functional regions of p125, a novel mammalian Sec23p-interacting protein. Biochem Biophys Res Commun. 2000 Dec 9;279(1):144-9. PubMed PMID: 11112430.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography using immobilized antigen.
Buffer System:
Stabilization buffer.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects a single 89 kDa protein in Jurkat cell extracts. It does not cross react with other proteins.
The antibody does immunoprecipitates solubilized Sec23 protein form Jurkat cell extracts.
Gene ID 10484

Accessory Products

Proteins and/or Positive Controls

Proteins for SEC23A (4 products)

Catalog No. Species Pres. Purity   Source  

SEC23A

SEC23A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

SEC23A

SEC23A Human Purified
  Abnova Taiwan Corp.

SEC23A

SEC23A Human
  Abnova Taiwan Corp.

SEC23A

SEC23A Human Purified
  Abnova Taiwan Corp.

Positive controls for SEC23A (2 products)

Catalog No. Species Pres. Purity   Source  

SEC23A Lysate

Western Blot: SEC23A Lysate [NBL1-15777] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SEC23A
  Novus Biologicals Inc.

SEC23A overexpression lysate

SEC23A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn