AP08460PU-N SET / I2PP2A (66-81, 135-151) antibody

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50 µg / €470.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Bovine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish SET / I2PP2A

Product Description for SET / I2PP2A

Rabbit anti Bovine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish SET / I2PP2A.
Properties: (66-81, 135-151)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for SET / I2PP2A

Product Category Primary Antibodies
Quantity 50 µg
Synonyms I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I
Presentation Aff - Purified
Reactivity Bov, Chk, Eq, Hst, Hu, Mky, Ms, Por, Rt, Xen, Ze
Applications P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
H0UI37
Immunogen:
A mixture of synthetic peptides corresponding to amino acids 66-81 and 135-151 of Human INHAT-1.
AA Sequence:
SET / TAF-I antibody was raised against synthetic peptides of amino acids 66-81 (PFFQKRSELIAKIPNF) and 135-151 (ENKVLSKEFHLNESGDP) of human INHAT-1.
GeneID:
100130890
Remarks:
Sequence homology is 100% in Mouse and Rat. Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%); Gorilla, Stickleback (94%); Dog (88%).
Property 66-81, 135-151
Application Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western blot: 1-2 µg/ml.
Background SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing: Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
Concentration 0.5 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography.
Buffer System:
PBS containing 0.1 mg/ml BSA as stabilizer and 0.02% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Bovine, Chicken, Xenopus, Hamster, Horse, Monkey, Pig, Zebrafish
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes SET/I2PP2A.
Gene ID 6418

Accessory Products

Proteins and/or Positive Controls

Proteins for SET / I2PP2A (6 products)

Catalog No. Species Pres. Purity   Source  

SET (1-290, His-tag)

SET Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

SET (1-290, His-tag)

SET Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

SET / I2PP2A

SET / I2PP2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SET / I2PP2A

SET / I2PP2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

TSTD3 (1-97, His-tag)

TSTD3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

TSTD3 (1-97, His-tag)

TSTD3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Positive controls for SET / I2PP2A (2 products)

Catalog No. Species Pres. Purity   Source  

SET Lysate

Western Blot: SET Lysate [NBL1-15867] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SET
0.1 mg / €400.00
  Novus Biologicals Inc.

TSTD3 overexpression lysate

TSTD3 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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