discontinued

AP15906PU-N SET / I2PP2A (N-term) antibody

See related secondary antibodies

Search for all "SET / I2PP2A"

0.1 mg / €340.00

Quick Overview

Goat anti Human SET / I2PP2A

Product Description for SET / I2PP2A

Goat anti Human SET / I2PP2A.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for SET / I2PP2A

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
H0UI37
Immunogen:
Peptide from the N Terminus of the protein sequence according to NP_003002.2
AA Sequence:
SAPAAKVSKKELNS-C
GeneID:
100130890
Property N-term
Application Peptide ELISA: 1/1000 (Limit Dilution). 
Western blot: 0.3-1 µg/ml. Approx 38kDa band observed in lysates of Human Kidney lysates and of cell lines Daudi and Molt4 (calculated MW of 32.1kDa according to NP_003002.2).
Background SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing: Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
Concentration 0.5 mg/ml
General Readings
  1. Ruiz PA, Kim SC, Sartor RB, Haller D. 15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity. J Biol Chem. 2004 Aug 20;279(34):36103-11. Epub 2004 Jun 15. PubMed PMID: 15199053.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Bovine (Cow)
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is expected to recognize isoform 2 (NP_003002.2).
Gene ID 6418

Accessory Products

Proteins and/or Positive Controls

Proteins for SET / I2PP2A (6 products)

Catalog No. Species Pres. Purity   Source  

SET (1-290, His-tag)

SET Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

SET (1-290, His-tag)

SET Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

SET / I2PP2A

SET / I2PP2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SET / I2PP2A

SET / I2PP2A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

TSTD3 (1-97, His-tag)

TSTD3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

TSTD3 (1-97, His-tag)

TSTD3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Positive controls for SET / I2PP2A (2 products)

Catalog No. Species Pres. Purity   Source  

SET Lysate

Western Blot: SET Lysate [NBL1-15867] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SET
0.1 mg / €400.00
  Novus Biologicals Inc.

TSTD3 overexpression lysate

TSTD3 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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