NBP1-78137 SHD antibody

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Rabbit anti Drosophila SHD


Product Description for SHD

Rabbit anti Drosophila SHD.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for SHD

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Dros
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Synthetic peptide taken form the Spook protein form within the mid-region peptide (from with in aa 175-225), the peptides were post-synthetically modified and conjugated to carrier protein to achieve desired antigenicity. The Shade peptides sequences are conserved various other specieis of fruit flys.
Background Transposable elements are found in the genomes of nearly all eukaryotes. The recent completion of the Release 3 euchromatic genomic sequence of Drosophila melanogaster by the Berkeley Drosophila Genome Project has provided precise sequence for the repetitive elements in the Drosophila euchromatin. The genomic sequence to describe the euchromatic transposable elements in the sequenced strain of this species identified several new genes. Out of 85 unknown and 8 novel family of transposable elements varying in copy number form 1-146 have been identified. A total of 1500 full and partial transposable elements were identified that make up about 4% of the fruit fly genome. Most of these transposable elements reside outside genes (1). Spook or cytochrome P450a1 enzymes are involved in the detoxification of alkaloids and in xenobiotics resistance in insects. Cytochrome P450s form a large and diverse family of heme-containing proteins capable of carrying out many different enzymatic reactions. Some P450 proteins carry out reaction that are essential for process such as hormone synthesis, while others are P450 enzymes are involved in detoxification of environmental compounds. Shade expression patterns in embryos and s stage of third instar larvae indicate the protein is in abundance in fat bodies, malpighian tubules and in distinct regions of the midgut, consistent with its role in detoxification (2). The biological functions also involve embryonic development via syncytical blastderm, ecdysone biosynthesis process, heat involution, cuticle development. Shade gene sequence location is at 3L:5583181..5585677 is a 540 aa (65kDa) protein. There are at least 14 alleles are reported for this gene. The D. melanogaster Shade-selective antibodies were generated against unique antigenic sequence taken form the N-terminal region with in the amino acids 1-60 and from 175-225. The sequence was conserved in several species of fruit flies, but was unique to shade/CYP341a1 protein and was not present in other proteins. The Shade antibodies were affinity purified over immobilized antigen based chromatography, and the affinity purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc., will provide limited quantities of antigenic blocking peptide for Shade antibodies.
Storage Store at -20C. Avoid freeze-thaw cycles.
Affinity purified
Aff - Purified
Gene ID 39592

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