AP05395D4-N Sheep/Goat IgG antibody

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Donkey anti Goat, Sheep Sheep/Goat IgG


Product Description for Sheep/Goat IgG

Donkey anti Goat, Sheep Sheep/Goat IgG.
Presentation: DyLight488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Sheep/Goat IgG

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation DyLight488
Reactivity Gt, Sh
Applications F, ICC/IF
Clonality Polyclonal
Host Donkey
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Sheep IgG
Application Immunofluorescence: 1/50-1/200.
Flow Cytometry: Use 50 µl of 1/50-1/200 diluted antibody to label 1x10e6 cells in 100 µl.
Concentration 1.0 mg/ml
General Readings
  1. Singh M, Cattaneo R, Billeter MA. A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice. J Virol. 1999 Jun;73(6):4823-8. PubMed PMID: 10233943. (Free PMC Article available, 4 images available)
  2. Tedla N, Wang HW, McNeil HP, Di Girolamo N, Hampartzoumian T, Wakefield D, et al. Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta. J Immunol. 1998 Nov 15;161(10):5663-72. PubMed PMID: 9820547.
  3. Turner J, Gonzalez-Juarrero M, Ellis DL, Basaraba RJ, Kipnis A, Orme IM, et al. In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice. J Immunol. 2002 Dec 1;169(11):6343-51. PubMed PMID: 12444141.
  4. Singh SK, Kurfurst R, Nizard C, Schnebert S, Perrier E, Tobin DJ. Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer. FASEB J. 2010 Oct;24(10):3756-69. doi: 10.1096/fj.10-159046. Epub 2010 May 25. PubMed PMID: 20501793.
  5. Yekta MA, Goddeeris BM, Vanrompay D, Cox E. Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding. Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):42-6. doi: 10.1016/j.vetimm.2010.11.010. Epub 2010 Nov 18. PubMed PMID: 21185088.
  6. Guitton C, Cottereau A, Gérard N, Quillard T, Chauveau A, Devallière J, et al. Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-κB, and ERK1/2 MAP kinases. Am J Physiol Cell Physiol. 2011 Apr;300(4):C833-42. doi: 10.1152/ajpcell.00003.2010. Epub 2011 Jan 12. PubMed PMID: 21228323.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Affinity Chromatography.
Buffer System:
Phosphate buffered saline pH 7.2 containing 0.09% Sodium Azide as preservative.
Liquid purified IgG fraction.
This antibody recognises both Sheep and Goat IgG, which are immunologically very similar.
This antibody is adsorbed against Human, Rabbit, Mouse and Rat serum to minimise cross-reactivity.
Sheep and Goat.

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