SM1514 Sheep Lambda light chain antibody

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Mouse anti Sheep Sheep Lambda light chain VPM8


Product Description for Sheep Lambda light chain

Mouse anti Sheep Sheep Lambda light chain VPM8.
Presentation: Supernatant
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for Sheep Lambda light chain

Product Category Primary Antibodies
Quantity 2 ml
Presentation Supernatant
Reactivity Sh
Applications E, F, IP, WB
Clonality Monoclonal
Clone VPM8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Sheep IgG. Cells from BALB/c mice were fused with cells of the NSO mouse myeloma cell line.
Application Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells.
Immunoprecipitation (Neat).
Immunohistochemistry on Frozen Sections (Neat-1/3).
Western blotting (1/10).
ELISA (1/10-1/500).
Background All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
General Readings
  1. Summers C, Neill W, Dewar P, Gonzalez L, van der Molen R, Norval M, et al. Systemic immune responses following infection with Jaagsiekte sheep retrovirus and in the terminal stages of ovine pulmonary adenocarcinoma. J Gen Virol. 2002 Jul;83(Pt 7):1753-7. PubMed PMID: 12075095.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Contains 0.2M Tris/HCl pH7.4 and 5-10% Foetal Calf Serum with 0.01% Sodium Azide as preservative.
Liquid Tissue Culture Supernatant
This antibody recognises ovine immunoglobulin lambda light chain.

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