AM26313BT-N Siglec-H antibody

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50 µg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse Siglec-H 440c

AM26313BT-N

Product Description for Siglec-H

Rat anti Mouse Siglec-H 440c.
Presentation: Biotin
Product is tested for Frozen Sections.

Properties for Siglec-H

Product Category Primary Antibodies
Quantity 50 µg
Presentation Biotin
Reactivity Ms
Applications C
Clonality Monoclonal
Clone 440c
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q3Y597
Isotype control SM19B
Application Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
Flow cytometry: The typical starting working dilution is 1:50.
Background Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen.
Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Concentration 0.1 mg/ml
General Readings
  1. Blasius AL, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood. 2006 Mar 15;107(6):2474-6. Epub 2005 Nov 17. PubMed PMID: 16293595. (Free PMC Article available, 2 images available)
  2. Blasius A, Vermi W, Krug A, Facchetti F, Cella M, Colonna M. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood. 2004 Jun 1;103(11):4201-6. Epub 2003 Dec 24. PubMed PMID: 14695235.
  3. Colonna, M et al; Plasmacytoid dendritic cells in immunity. Nat Immunol 2004, 5: 1219.
  4. Seth S, Oberdörfer L, Hyde R, Hoff K, Thies V, Worbs T, et al. CCR7 essentially contributes to the homing of plasmacytoid dendritic cells to lymph nodes under steady-state as well as inflammatory conditions. J Immunol. 2011 Mar 15;186(6):3364-72. doi: 10.4049/jimmunol.1002598. Epub 2011 Feb 4. PubMed PMID: 21296980.
Storage

Store at 2 - 8 °C.
Shelf life: one year from despatch.

Format
Label:
Biotin
Purification:
Protein G
Buffer System:
PBS
Preservatives:
0.02% sodium azide
Stabilizers:
0.1% bovine serum albumin
State:
Liquid 0.2 µm filtered Ig fraction
Species Reactivity
Species reactivity (tested):
Mouse
Specificity
Specificity:
The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC).

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