discontinued

AM32892PU-N Siglec-H (286-304) antibody

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0.5 mg / €330.00

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Rat anti Mouse Siglec-H IMG23M14A10

AM32892PU-N

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  • AM32892PU-N

Product Description for Siglec-H

Rat anti Mouse Siglec-H IMG23M14A10.
Properties: (286-304)
Presentation: Purified
Product is tested for Paraffin Sections, Flow Cytometry, Western blot / Immunoblot.

Properties for Siglec-H

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Reactivity Ms
Applications F, P, WB
Clonality Monoclonal
Clone IMG23M14A10
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q3Y597
Immunogen:
A portion of amino acids 275-325 of Mouse Siglec-H
Property 286-304
Isotype control SM15P, SM15PX
Application Flow Cytometry (CS): 1 µg/106 cells. 
Western blot analysis: 3-5 µg/ml
Immunohistochemistry on Paraffin Sections: 5 μg/ml.
Background Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2).  Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responses.  This leads to lessened expansion and Th1/Th17 polarization (3).  Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance.  A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness.  Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects (4, 5).  Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.
Concentration 0.5 mg/ml
General Readings
  1. Apostolou I, von Boehmer H. In vivo instruction of suppressor commitment in naive T cells. J Exp Med. 2004 May 17;199(10):1401-8. PubMed PMID: 15148338. (Free PMC Article available, 5 images available)
  2. Kang HK, Liu M, Datta SK. Low-dose peptide tolerance therapy of lupus generates plasmacytoid dendritic cells that cause expansion of autoantigen-specific regulatory T cells and contraction of inflammatory Th17 cells. J Immunol. 2007 Jun 15;178(12):7849-58. PubMed PMID: 17548623.
  3. Zhang J, Raper A, Sugita N, Hingorani R, Salio M, Palmowski MJ, et al. Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors. Blood. 2006 May 1;107(9):3600-8. Epub 2006 Jan 5. PubMed PMID: 16397130.
  4. Loschko, J et al. 2011 Antigen targeting to Plasmycotid Dendritic Cells via Siglec-H inhibits Th-Cell-Independent Autoimmunity. J. Immunol 187 doi: 10-4019/jimmunol. 11 02307.
  5. http://www.copewithcytokines.de/cope.cgi?key=SIGLEC-H__REFERENCES
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody recognizes Mouse Siglec-H.
Other species not tested.

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