AM32892RP-N Siglec-H (286-304) antibody

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Rat anti Mouse Siglec-H IMG23M14A10

Product Description for Siglec-H

Rat anti Mouse Siglec-H IMG23M14A10.
Properties: (286-304)
Presentation: PE
Product is tested for Flow Cytometry.

Properties for Siglec-H

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation PE
Reactivity Ms
Applications F
Clonality Monoclonal
Clone IMG23M14A10
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
A portion of amino acids 275-325 of Mouse Siglec-H.
Property 286-304
Isotype control SM15P, SM15PX
Application Flow Cytometry (CS): Use at 1 μg/106 cells.
Background Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2).  Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responses.  This leads to lessened expansion and Th1/Th17 polarization (3).  Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance.  A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness.  Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects (4, 5).  Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.
Concentration 0.5 mg/ml
General Readings
  1. Blasius AL, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood. 2006 Mar 15;107(6):2474-6. Epub 2005 Nov 17. PubMed PMID: 16293595. (Free PMC Article available, 2 images available)
  2. Apostolou I, von Boehmer H. In vivo instruction of suppressor commitment in naive T cells. J Exp Med. 2004 May 17;199(10):1401-8. PubMed PMID: 15148338. (Free PMC Article available, 5 images available)
  3. Kang HK, Liu M, Datta SK. Low-dose peptide tolerance therapy of lupus generates plasmacytoid dendritic cells that cause expansion of autoantigen-specific regulatory T cells and contraction of inflammatory Th17 cells. J Immunol. 2007 Jun 15;178(12):7849-58. PubMed PMID: 17548623.
  4. Zhang J, Raper A, Sugita N, Hingorani R, Salio M, Palmowski MJ, et al. Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors. Blood. 2006 May 1;107(9):3600-8. Epub 2006 Jan 5. PubMed PMID: 16397130.
  5. Loschko, J et al. 2011 Antigen targeting to Plasmycotid Dendritic Cells via Siglec-H inhibits Th-Cell-Independent Autoimmunity. J. Immunol 187 doi: 10-4019/jimmunol. 11 02307.
  6. http://www.copewithcytokines.de/cope.cgi?key=SIGLEC-H__REFERENCES
  7. Lau-Kilby AW, Kretz CC, Pechhold S, Price JD, Dorta S, Ramos H, et al. Interleukin-2 inhibits FMS-like tyrosine kinase 3 receptor ligand (flt3L)-dependent development and function of conventional and plasmacytoid dendritic cells. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2408-13. doi: 10.1073/pnas.1009738108. Epub 2011 Jan 24. PubMed PMID: 21262836. (Free PMC Article available, 7 images available)
  8. Gregorio J, Meller S, Conrad C, Di Nardo A, Homey B, Lauerma A, et al. Plasmacytoid dendritic cells sense skin injury and promote wound healing through type I interferons. J Exp Med. 2010 Dec 20;207(13):2921-30. doi: 10.1084/jem.20101102. Epub 2010 Nov 29. PubMed PMID: 21115688. (Free PMC Article available, 6 images available)
  9. Andrews DM, Estcourt MJ, Andoniou CE, Wikstrom ME, Khong A, Voigt V, et al. Innate immunity defines the capacity of antiviral T cells to limit persistent infection. J Exp Med. 2010 Jun 7;207(6):1333-43. doi: 10.1084/jem.20091193. Epub 2010 May 31. PubMed PMID: 20513749. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C.
This products is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Protein G Chromatography
Buffer System:
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction
This antibody recognizes Mouse Siglec-H. 
Other species not tested.

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