AM60041FC-N SIGLEC5 / SIGLEC14 antibody

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0.1 mg / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Chimpanzee, Human SIGLEC5 / SIGLEC14 1A5

Product Description for SIGLEC5 / SIGLEC14

Mouse anti Chimpanzee, Human SIGLEC5 / SIGLEC14 1A5.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for SIGLEC5 / SIGLEC14

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms CD170, O15389, Q08ET2, Sialic acid-binding Ig-like lectin 14, Sialic acid-binding Ig-like lectin 5
Presentation FITC
Reactivity Chimp, Hu
Applications F
Clonality Monoclonal
Clone 1A5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1
Isotype control AM03095PU-N, SM10F (for use in human samples)
Application Flow cytometry: Neat-1/10, use 10µl of this working dilution to label 10e6 cells in 100µl.
Background Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.
Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases.
Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.
Concentration 0.1 mg/ml
General Readings
  1. Cornish AL, Freeman S, Forbes G, Ni J, Zhang M, Cepeda M, et al. Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33. Blood. 1998 Sep 15;92(6):2123-32. PubMed PMID: 9731071.
  2. Avril T, Freeman SD, Attrill H, Clarke RG, Crocker PR. Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. J Biol Chem. 2005 May 20;280(20):19843-51. Epub 2005 Mar 15. PubMed PMID: 15769739.
  3. Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A. Loss of Siglec expression on T lymphocytes during human evolution. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7765-70. Epub 2006 May 8. PubMed PMID: 16682635. (Free PMC Article available, 7 images available)
  4. Jaroenpool J, Rogers KA, Pattanapanyasat K, Villinger F, Onlamoon N, Crocker PR, et al. Differences in the constitutive and SIV infection induced expression of Siglecs by hematopoietic cells from non-human primates. Cell Immunol. 2007 Nov-Dec;250(1-2):91-104. doi: 10.1016/j.cellimm.2008.01.009. Epub 2008 Mar 10. PubMed PMID: 18331725. (Free PMC Article available, 6 images available)
  5. Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M. Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J. 2006 Oct;20(12):1964-73. PubMed PMID: 17012248.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium azide and 1% BSA
State:
Liquid purified IgG
Purified
Species Reactivity
Species reactivity (expected):
Chimpanzee (Nguyen, D.H. et al., 2006)
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody recognises human SIGLEC5 / CD170.  Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).

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