discontinued

AM05887PU-N SLA CLASS II DR antibody

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0.25 mg / €520.00

Quick Overview

Mouse anti Bovine, Porcine SLA CLASS II DR 2E9/13

AM05887PU-N

Product Description for SLA CLASS II DR

Mouse anti Bovine, Porcine SLA CLASS II DR 2E9/13.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for SLA CLASS II DR

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms MHC Class II DR
Presentation Purified
Reactivity Bov, Por
Applications C, F, IP
Clonality Monoclonal
Clone 2E9/13
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Porcine monocytes
Isotype control AM03110PU-N, SM12P
Application Immunohistochemistry on frozen sections.
Immunoprecipitation: The antibody immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells.
Flow Cytometry: 1/10, use 10 µl of the suggested working dilution to 1x10e6 cells in 100 µl.
Functional assays: Clone 2E9/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B (1).
Concentration 1.0 mg/ml
General Readings
  1. Bullido R, Doménech N, Alvarez B, Alonso F, Babín M, Ezquerra A, et al. Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals. Dev Comp Immunol. 1997 May-Jun;21(3):311-22. PubMed PMID: 9258612.
Storage Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
Phosphate buffered saline pH 7.4, 0.09% Sodium Azide
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Liquid Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for porcine MHC class II DR molecules (SLA-II DR). Porcine SLA-II molecules are expressed on all B cells, on antigen presenting cells and on certain subsets of resting and activated T cells.
This clone reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognises a monomorphic determinant of porcine SLA-II DR.
Species:
Pig, Bovine.

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