AM05887PU-S SLA CLASS II DR antibody

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0.1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Porcine SLA CLASS II DR 2E9/13

Product Description for SLA CLASS II DR

Mouse anti Bovine, Porcine SLA CLASS II DR 2E9/13.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for SLA CLASS II DR

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms MHC Class II DR
Presentation Purified
Reactivity Bov, Por
Applications C, F, IP
Clonality Monoclonal
Clone 2E9/13
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Porcine monocytes.
Application Flow Cytometry: Use 10 μl of 1/25-1/200 diluted antibody to label 106 cells in 100 μl. 
Immunoprecipitation: This antibody immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells. 
Immunohistochemistry on Frozen Sections.
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.
Concentration 1.0 mg/ml
General Readings
  1. Bullido R, Doménech N, Alvarez B, Alonso F, Babín M, Ezquerra A, et al. Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals. Dev Comp Immunol. 1997 May-Jun;21(3):311-22. PubMed PMID: 9258612.
  2. Jeong HJ, Song YJ, Lee SW, Lee JB, Park SY, Song CS, et al. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol. 2010 Apr;17(4):503-12. doi: 10.1128/CVI.00365-09. Epub 2010 Feb 3. PubMed PMID: 20130128. (Free PMC Article available, 8 images available)
  3. Ding Q, Lu L, Zhou X, Zhou Y, Chou KY. Human PD-L1-overexpressing porcine vascular endothelial cells induce functionally suppressive human CD4+CD25hiFoxp3+ Treg cells. J Leukoc Biol. 2011 Jul;90(1):77-86. doi: 10.1189/jlb.1210691. Epub 2011 Apr 15. PubMed PMID: 21498584.
  4. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update. Vet Res. 39: 54.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Affinity Chromatography on Protein G
Buffer System:
0.09% Sodium Azide
Liquid purified IgG fraction
Clone 2E9/13 is specific for SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. 
This clone reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognises a monomorphic determinant of porcine SLA DR. Clone 2E9/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B, see Bullido, R. et al. for details.
Pig, Bovine.

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